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首页> 外文期刊>Biotechnology Progress >Invariability of Central Metabolic Flux Distribution in Shewanella oneidensis MR-1 Under Environmental or Genetic Perturbations
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Invariability of Central Metabolic Flux Distribution in Shewanella oneidensis MR-1 Under Environmental or Genetic Perturbations

机译：环境或遗传扰动下沙瓦氏假单胞菌MR-1中央代谢通量分布的不变性

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摘要

An environmentally important bacterium with versatile respiration, Shewanella oneidensis MR-1, displayed significantly different growth rates under three culture conditions: minimal medium (doubling time ~3 h), salt stressed minimal medium (doubling time ~6 h), and minimal medium with amino acid supplementation (doubling time ~1.5 h). ~(13)C-based metabolic flux analysis indicated that fluxes of central metabolic reactions remained relatively constant under the three growth conditions, which is in stark contrast to the reported significant changes in the transcript and metabolite profiles under various growth conditions. Furthermore, 10 transposon mutants of S. oneidensis MR-1 were randomly chosen from a transpo-son library and their flux distributions through central metabolic pathways were revealed to be identical, even though such mutational processes altered the secondary metabolism, for example, glycine and C1 (5,10-Me-THF) metabolism.
机译：具有多种呼吸作用的环境重要细菌Shewanella oneidensis MR-1在三种培养条件下显示出明显不同的生长速率：最小培养基（加倍时间〜3 h），盐胁迫最小培养基（加倍时间〜6 h）和最小培养基氨基酸补充（加倍时间〜1.5小时）。基于（13）C的代谢通量分析表明，在三种生长条件下，中心代谢反应的通量保持相对恒定，这与报道的在各种生长条件下转录本和代谢物谱的显着变化形成鲜明对比。此外，从转座子文库中随机选择了10个转座子沙门氏菌MR-1突变体，通过中枢代谢途径的通量分布被揭示是相同的，即使这种突变过程改变了次级代谢，例如甘氨酸和C1（5,10-Me-THF）代谢。

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