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首页> 外文期刊>Biotechnology Progress >Ferrous Iron Oxidation by Foam Immobilized Acidithiobacillus ferrooxidans: Experiments and Modeling
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Ferrous Iron Oxidation by Foam Immobilized Acidithiobacillus ferrooxidans: Experiments and Modeling

机译：泡沫固定的酸性氧化硫硫亚铁氧化亚铁对亚铁的氧化作用：实验与建模

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摘要

Ferrous iron bio-oxidation by Acidithiobacillus ferrooxidans immobilized on polyurethane foam was investigated. Cells were immobilized on foams by placing them in a growth environment and fully bacterially activated polyurethane foams (BAPUFs) were prepared by serial subculturing in batches with partially bacterially activated foam (pBAPUFs). The dependence of foam density on cell immobilization process, the effect of pH and BAPUF loading on ferrous oxidation were studied to choose operating parameters for continuous operations. With an objective to have high cell densities both in foam and the liquid phase, pretreated foams of density 50 kg/m~3 as cell support and ferrous oxidation at pH 1.5 to moderate the ferric precipitation were preferred. A novel basket-type bioreactor for continuous ferrous iron oxidation, which features a multiple effect of stirred tank in combination with recirculation, was designed and operated. The results were compared with that of a free cell and a sheet-type foam immobilized reactors. A fivefold increase in ferric iron productivity at 33.02 g/h/L of free volume in foam was achieved using basket-type bioreactor when compared to a free cell continuous system. A mathematical model for ferrous iron oxidation by Acidithiobacillus ferrooxidans cells immobilized on polyurethane foam was developed with cell growth in foam accounted by an effectiveness factor. The basic parameters of simulation were estimated using the experimental data on free cell growth as well as from cell attachment to foam under nongrowing conditions. The model predicted the phase of both oxidation of ferrous in shake flasks by pBAPUFs as well as by fully activated BAPUFs for different cell loadings in foam. Model for stirred tank basket bioreactor predicted within 5% both transient and steady state of the experiments closely for the simulated dilution rates. Bio-oxidation at high Fe~(2+) concentrations were simulated with experiments when substrate and product inhibition coefficients were factored into cell growth kinetics.
机译：研究了固定在聚氨酯泡沫塑料上的氧化亚铁硫杆菌对亚铁的生物氧化作用。通过将细胞置于生长环境中，将细胞固定在泡沫上，并通过与部分细菌活化的泡沫（pBAPUF）分批连续传代制备完全细菌活化的聚氨酯泡沫（BAPUF）。研究了泡沫密度对细胞固定过程的依赖性，pH和BAPUF负载量对亚铁氧化的影响，以选择连续操作的操作参数。为了在泡沫和液相中均具有高的泡孔密度，优选密度为50kg / m 3的预处理泡沫作为泡孔载体并在pH 1.5下进行亚铁氧化以缓和铁的沉淀。设计并运行了一种新型的用于亚铁连续氧化的篮式生物反应器，该反应器具有搅拌槽与再循环相结合的多重作用。将结果与自由室和片状泡沫固定反应器的结果进行比较。与自由单元连续系统相比，使用篮式生物反应器在泡沫中的自由体积为33.02 g / h / L时，铁的生产率提高了五倍。建立了固定在聚氨酯泡沫上的氧化亚铁硫杆菌氧化亚铁细胞氧化亚铁的数学模型，其中泡沫的细胞生长是由有效因子引起的。使用关于自由细胞生长以及在非生长条件下从细胞附着到泡沫的实验数据估计了模拟的基本参数。该模型预测了pBAPUFs和完全活化的BAPUFs对摇瓶中亚铁的氧化阶段，以适应泡沫中不同的细胞负载。搅拌罐篮式生物反应器的模型在模拟的稀释速率下，可在5％的瞬态和稳态下精确地预测实验。当底物和产物抑制系数纳入细胞生长动力学时，通过实验模拟了高Fe〜（2+）浓度下的生物氧化。

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