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首页> 外文期刊>World Mycotoxin Journal >Correlation and variability between weighing, counting and analytical methods to determine ergot (Claviceps purpurea) contamination of grain
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Correlation and variability between weighing, counting and analytical methods to determine ergot (Claviceps purpurea) contamination of grain

机译：称重，计数和分析方法之间的相关性和可变性，以确定麦片（Claviceps Purpurea）粒度污染

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摘要

Ergot alkaloid mycotoxins produced by the fungus Claviceps purpurea, are contaminants of cereal crops and grasses. The objectives of this study were to determine the correlation between number of ergot sclerotia and weight compared to the total ergot alkaloid concentration, to evaluate the effect of grinding process (i.e. particle size (PS)) on ergot alkaloid analysis using high performance liquid chromatography - tandem mass spectrometry, and to determine the impact of sample volume on analytical variability. This study demonstrated that correlations exist between both ergot sclerotia count (R-2=0.7242, P<0.001) and ergot sclerotia weight (R-2=0.9618, P<0.001) compared to the total alkaloid concentration of 6 ergot alkaloids. However, at alkaloid ergot concentrations below 350 aeg/kg grain, ergot sclerotia count (R-2=0.0002, P=0.956) and ergot sclerotia weight (R-2=0.0064, P=0.769) were not correlated to the total alkaloid concentration. A lower variability (P=0.041), defined by coefficient of variation (CV), was observed using a commercial UDY cyclone sample mill (PS=192 mu m, CV= 9 aeg/kg) as compared to a household coffee grinder (PS=516 mu m, CV= 66 aeg/kg). Total amount and concentration of individual ergot alkaloids varied (P<0.05) among sclerotia of similar weight. For the analytical method, CV was numerically reduced as sample volume increased (97% CV for 75 ml to 64% CV for 1000 ml; mean of all concentrations) but increased as sample concentration declined (17% CV for 81,678 mu g/kg to 284% for 35 mu g/kg; mean of all sample volumes). This implies that analysis of small sample volumes at low ergot alkaloid concentrations may result in highly variable and potentially misleading results. In conclusion, number of ergot sclerotia and weight are unreliable indicators of alkaloid content at ergot concentrations below 350 aeg/kg and particle size influences the variability. An analytical approach with fine grinding (mean PS<200 mu m, 85% particles <400 mu m) of a large sample should be used to assess low-level ergot contamination.
机译：用真菌克拉夫莫斯紫癜生产的麦耳生物碱霉菌毒素是谷物作物和草的污染物。本研究的目的是确定与总麦格白序浓度相比的麦格白核苷酸和重量之间的数量与重量之间的相关性，以评估使用高效液相色谱法对磨削过程的影响（即粒度（PS）） - 串联质谱，并确定样品体积对分析变异性的影响。该研究表明，与6个麦角醛类生物碱的总生物碱浓度相比，ERGOT菌核计数（R-2 = 0.7242，P <0.001）和ERGOT肠菌重量（R-2 = 0.9618，P <0.001）之间存在相关性。但是，在低于350 AEG / kg晶粒的生物碱麦耳浓度下，Ergot Sclerotia Count（R-2 = 0.0002，P = 0.956）和Ergot Scolotia重量（R-2 = 0.0064，P = 0.769）与总生物碱浓度无关。与家用咖啡研磨机相比，使用商业UDY旋风器样品轧机（PS =192μm，CV = 9 AEG / kg）观察通过变异系数（CV）而定义的较低的变异性（P = 0.041）。相比，与家用咖啡研磨机（PS = 516 mu m，cv = 66 aeg / kg）。单个麦耳生物碱的总量和浓度不同重量的核苷酸中的（P <0.05）。对于分析方法，随着样品体积增加（97％CV至64％CV为1000mL的97％CV为1000mL的均值），但随着样品浓度下降（17％CV，17％CV为81,678μg/ kg的平均值，CV为数量，但随着样品体积为含量，但随着样品浓度的增加（17％CV为81,678μg/ kg，为所有浓度），但增加35 mu g / kg的284％;所有样本量的平均值）。这意味着在低麦角生物碱浓度下对小样品体积的分析可能导致高度可变和潜在的误导性结果。总之，Ergot肠菌和重量的数量是低于350 AEG / kg的麦格麦​​碱含量的不可靠指标，并且粒度影响变异性。应使用大型研磨的微型研磨（平均pS <200μm，85％颗粒<400μm）的分析方法来评估低水平的麦角污染。

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  • 来源
    《World Mycotoxin Journal》 |2017年第3期|共10页
  • 作者

    Grusie T.; Cowan V.; Singh J.; McKinnon J.; Blakley B.;

  • 作者单位

    Univ Saskatchewan Western Coll Vet Med 52 Campus Dr Saskatoon SK Canada;

    Univ Saskatchewan Western Coll Vet Med 52 Campus Dr Saskatoon SK Canada;

    Univ Saskatchewan Western Coll Vet Med 52 Campus Dr Saskatoon SK Canada;

    Univ Saskatchewan Dept Anim &

    Poultry Sci 51 Campus Dr Saskatoon SK Canada;

    Univ Saskatchewan Western Coll Vet Med 52 Campus Dr Saskatoon SK Canada;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    sclerotia; sample volume; particle size;

    机译：巩膜;样品体积;粒度;

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