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首页> 外文期刊>Biotechnology Progress >Partitioning of Amino Acids in the Aqueous Biphasic System Containing the Water-Miscible Ionic Liquid 1-Butyl-3-Methylimidazolium Bromide and the Water-Structuring Salt Potassium Citrate
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Partitioning of Amino Acids in the Aqueous Biphasic System Containing the Water-Miscible Ionic Liquid 1-Butyl-3-Methylimidazolium Bromide and the Water-Structuring Salt Potassium Citrate

机译：氨基酸在包含水混溶性离子液体1-丁基-3-甲基咪唑鎓溴化物和水结构化柠檬酸钾的双相水体系中的分配

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In biotechnology, extraction by means of aqueous biphasic systems (ABS) is known as a promising tool for the recovery and purification of bio-molecules. Over the past decade, the increasing emphasis on cleaner and environmentally benign extraction procedures has led to enhanced interest in the ABS containing ionic liquids (ILs)-a new class of non-volatile alternative solvents. ABS composed of the hydrophilic IL (1-butyl-3-methylimidazolium bromide ([C4m]Br)} and potassium citrate-which is easily degraded-represents a clean media to green separation of bio-molecules. In this regard, here, the extraction capability of this ABS was evaluated through its application to the extraction of some amino acids. To gain an insight into the driving forces of amino acid partitioning in the studied It-based ABS, the distribution of five model amino acids (L-tryptophan, L-phenylalanine, L-tyrosine, L-leucine, and L-valine) at different aqueous medium pH values and different phase compositions was investigated. The studies indicated that hydrophobic interactions were the main driving force, although electrostatic interactions and salting-out effects were also important for the transfer of the amino acids. Moreover, based on the statistical analysis of the driving forces of amino acid partitioning in the studied IL-based ABS, a model was established to describe the partition coefficient of three model amino acids, L-tryptophan, L-phenylalanine, and L-valine, and employed to predict the partition coefficient of two other model amino acids, L-tyrosine and L-leucine.
机译：在生物技术中，借助于含水双相系统（ABS）进行提取被认为是用于回收和纯化生物分子的有前途的工具。在过去的十年中，对清洁和对环境无害的提取程序的日益重视导致人们对含ABS的离子液体（ILs）（一种新型的非挥发性替代溶剂）的兴趣增强。由亲水性IL（1-丁基-3-甲基咪唑鎓溴化物[[C4m] Br）}和柠檬酸钾（易于降解）组成的ABS代表了绿色分子生物分离的干净介质。通过将其用于某些氨基酸的提取，评估了该ABS的提取能力。为了深入了解所研究的基于It的ABS中氨基酸分配的驱动力，共有五个模型氨基酸（L-色氨酸，研究了在不同水介质pH值和不同相组成下的L-苯丙氨酸，L-酪氨酸，L-亮氨酸和L-缬氨酸，研究表明，尽管静电作用和盐析作用，疏水作用是主要驱动力对氨基酸的转移也很重要，而且，基于对基于IL的ABS中氨基酸分配的驱动力的统计分析，建立了描述分配的模型L-色氨酸，L-苯丙氨酸和L-缬氨酸三个模型氨基酸的系数，并用于预测其他两个模型氨基酸L-酪氨酸和L-亮氨酸的分配系数。

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