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首页> 外文期刊>Journal of land use science >Degumming Crude Enzyme Produced by Bacillus cereus HDYM-02 and its Application in Flax Retting
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Degumming Crude Enzyme Produced by Bacillus cereus HDYM-02 and its Application in Flax Retting

机译：由芽孢杆菌HDYM-02生产的脱胶粗酶及其在Flax Retting中的应用

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摘要

A cellulase-free, degumming crude enzyme was produced by Bacillus cereus HDYM-02, using konjaku flour as an inexpensive substrate. After 48 h fermentation, this crude enzyme consisted of pectinase and mannanase, whose maximum activity was 756.7 U/mL and 2967.3 U/mL, respectively. This crude enzyme exhibited considerable stability under the conditions resembling industrial flax retting. After 120 h incubation, more than 50% of the maximum activity of both pectinase and mannanase was retained at pH value 4.0 to 7.0, and at least 70% of the maximum activity was detected at 25 degrees C to 40 degrees C. The degumming liquid retted by this crude enzyme contained more galacturonic acid and reducing sugar than those in the degumming liquid retted by commercial pectinase. The application of B. cereus HDYM-02 crude enzyme resulted in higher weight loss of flax stems, better properties, higher productivities, and smoother surfaces of flax fibres. This study showed promise for the use of B. cereus HDYM-02 crude enzyme for flax retting in the textile industry.
机译：通过芽孢杆菌HDYM-02制备无纤维素酶的脱胶粗酶，以康雅粉作为廉价的基材。在发酵48小时后，该粗酶由果胶酶和甘露糖酶组成，其最大活性分别为756.7u / ml和2967.3u / ml。该粗酶在类似于工业亚麻保留的条件下表现出相当大的稳定性。孵育120小时后，将果胶酶和甘露烷酶的最大活性的超过50％保留在pH值4.0至7.0，并且至少70％的最大活性在25℃至40℃下检测到脱胶液体由该粗酶重新含有更多的半乳糖醛酸和还原糖，而不是商业果胶酶重新脱胶液中的降低糖。 B.Cereus HDYM-02粗酶的应用导致亚麻茎的体重减轻，更好的性能，更高的生产性，以及亚麻纤维的光滑表面。本研究表明，使用B.Cereus HDYM-02粗酶用于在纺织工业中使用B. Cereus HDYM-02粗酶。

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