首页> 外文期刊>Journal of Korean Forest Society >Effect of Nitrogen on Eleutheroside production and Adventitious Root Growth in Eleutherococcus koreanum Nakai Bioreactor Cultures
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Effect of Nitrogen on Eleutheroside production and Adventitious Root Growth in Eleutherococcus koreanum Nakai Bioreactor Cultures

机译:氮对eleuthercocccus的eleutheroside生产和不定根生长的影响

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This study was carried out to investigate the effect of NO_3 and NH_4~+ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus koreanum in 5 L-bioreactor culture. The change in the medium components was also measured during culture. The fresh weignt of adventitious root reached to the highest level. of 30.8 g FW/L in the presence of both 50 mM NO_3~- and 10 mM NH_4~+, representing 3.6-fold increase compared to the 60 mM NH_4~+ alone. However, as the increase of the portion of NH_4~+, the root growth was decreased. However, the maximum eleutheroside B, E and El contents were 57.3 (ig/g DW, 188.4 ug/g DW and 47.3 mu g/g DW, with 30 mM, 60 mM and 15 mM total nitrogen source, respectively. Fresh weight of adventitious root increased up to 6.8-fold of inoculum size within 9 weeks. The amounts of NH_4~+ K~+ NO_3~- and PO_4~- were decreased during culture periods. Based on these results, we suggest that various further studies are required to increase the biomass and the useful secondary metabilites.
机译:本研究进行了探讨NO_3和NH_4〜+对5L-生物反应器培养物中的左右肠球菌和eLeuthroside合成的疗效。在培养过程中也测量培养基组分的变化。不定根的新鲜威力达到最高水平。在50 mm NO_3〜 - 和10 mm NH_4〜+的情况下,30.8g fw / l,与单独的60 mm NH_4〜+相比,表示3.6倍的增加。然而,随着NH_4〜+部分的增加,根生长减少。然而,最大eleutheroside B,E和EL含量为57.3(Ig / g DW,188.4ug / g dw和47.3μg/ g dw,分别为30mm,60mm和15mm的总氮源。鲜重不定根在9周内增加6.8倍的接种尺寸。在培养期间,NH_4〜+ k〜+ NO_3〜 - 和PO_4〜 - 在这些结果中,我们建议需要各种进一步研究增加生物质和有用的二级核石。

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