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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Listeria monocytogenes Listeria monocytogenes infection differentially affects expression of ligands for NK cells and NK cell responses, depending on the cell type infected
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Listeria monocytogenes Listeria monocytogenes infection differentially affects expression of ligands for NK cells and NK cell responses, depending on the cell type infected

机译：李斯特菌单核细胞增生李斯特菌李斯特菌单核细胞增生感染差异地影响NK细胞和NK细胞应答的配体的表达，这取决于感染的细胞类型

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Abstract The pivotal role of NK cells in viral infection is extensively studied, whereas the role of NK cells in bacterial infection has been poorly investigated. Here, we have examined how Listeria monocytogenes (LM) affects expression of ligands for NK cell receptors and subsequent NK cell responses, depending on the type of cell infected. LM infected rat cell lines derived from different tissues were coincubated with splenic NK cells, and NK cell proliferation and IFN‐γ production were measured. In addition, expression of ligands for the NK cell receptors Ly49 and NK cell receptor protein 1 (NKR‐P1), MHC class I and C‐type lectin‐related molecules, respectively, was assessed. Infected pleural R2 cells, but not epithelium‐derived colon carcinoma cell line CC531 cells, induced proliferation of NK cells. Reporter cells expressing the inhibitory NKR‐P1G receptor or the activating NKR‐P1F receptor were less stimulated under incubation with infected CC531 cells versus uninfected CC531 controls, suggesting that the ligand(s) in question were down‐regulated by infection. Conversely, LM infection of R2 cells did not affect reporter cell stimulation compared with uninfected R2 controls. We characterized a rat monocyte cell line, termed RmW cells. In contrast to LM infected R2 cells that up‐regulate MHC class I molecules, RmW cells displayed unchanged MHC class I expression following infection. In line with MHC class I expression, more NK cells produced a higher amount of IFN‐γ against infected R2 cells compared with RmW cells. Together, L. monocytogenes infection may variously regulate cellular ligands for NK cells, depending on the cell type infected, affecting the outcome of NK cell responses.
机译：摘要广泛研究了NK细胞在病毒感染中的关键作用，而NK细胞在细菌感染中的作用也很难研究。在这里，我们研究了如何影响NK细胞受体和随后的NK细胞应答的配体的表达的影响，这取决于感染的细胞的类型。 LM感染的大鼠衍生自不同组织的大鼠细胞系用脾细胞均匀，测量NK细胞增殖和IFN-γ产生。此外，还评估NK细胞受体Ly49和NK细胞受体蛋白1（NKR-P1），MHC I类和C型凝集素相关分子的配体的表达。感染胸腔R2细胞，但不是上皮衍生的结肠癌细胞系CC531细胞，诱导NK细胞的增殖。表达抑制性NKR-P1G受体或激活的NKR-P1F受体的报告细胞在与感染的CC531细胞的温育下诱导较少刺激，而是未感染的CC531对照，表明有问题的配体被感染下调。相反，与未感染的R2对照相比，R2细胞的LM感染不会影响报告细胞刺激。我们表征了大鼠单核细胞细胞系，称为RMW细胞。与LM感染的R2细胞相比，上调MHC I类分子，RMW细胞显示出在感染后未改变的MHC I类表达。与MHC I类表达相比，与RMW细胞相比，更多NK细胞对受感染的R2细胞产生较高量的IFN-γ。 L.单核细胞增生感染可以各种调节NK细胞的细胞配体，这取决于感染的细胞类型，影响NK细胞应答的结果。

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