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Silver spray deposition for AgLDI imaging MS of cholesterol and other olefins on thin tissue sections

机译：用于Agldi成像胆固醇和其他烯烃在薄组织部分上的银喷雾沉积

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Abstract >Olefins such as cholesterol and unsaturated fatty acids play important biological roles. Silver‐assisted laser desorption ionization (AgLDI) takes advantage of the strong affinity of silver to conjugate with double bonds to selectively ionize these molecules for imaging mass spectrometry (IMS) experiments. For IMS studies, two main approaches for silver deposition have been described in the literature: fine coating by silver sputtering and spray deposition of silver nanoparticles. While these approaches allow for extremely high resolution IMS experiments to be conducted, they are not readily available to all laboratories. Herein, we present a silver nitrate spray deposition approach as an alternative to silver sputtering and nanoparticle deposition for routine IMS analysis. The silver nitrate spray has the same level of specificity and sensitivity for olefins, particularly cholesterol, and has shown to be capable of IMS experiments down to 10‐μm spatial resolution. Minimal sample preparation and the affordability of silver nitrate make this a convenient and accessible technique worth considering. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >烯烃如胆固醇和不饱和脂肪酸起着重要的生物作用。银辅助激光解吸电离（AgLDI）利用银与双键的缀合物的强亲和力，以选择性地电离这些分子用于成像质谱（IMS）实验。对于IMS研究，文献中已经描述了两种主要的银沉积方法：通过银溅射和喷涂银纳米颗粒的喷涂。虽然这些方法允许极高的分辨率IMS实验进行，但所有实验室都没有易于使用。在此，我们呈硝酸银喷雾沉积方法作为银溅射和纳米颗粒沉积的替代，用于常规IMS分析。硝酸银喷雾具有相同水平的烯烃，特别是胆固醇的特异性和敏感性，并且已经表明能够将IMS实验降低至10μm的空间分辨率。最小的样品制备和硝酸银的可承受能力使得这是一种值得考虑的方便和可访问的技术。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2020年第4期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Ethan&option=202" target="_blank" rel="nofollow">Yang Ethan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fournelle Frédéric&option=202" target="_blank" rel="nofollow">Fournelle Frédéric;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chaurand Pierre&option=202" target="_blank" rel="nofollow">Chaurand Pierre;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryUniversity of MontrealMontreal Quebec Canada H3C 3J7;</p> <p>Department of ChemistryUniversity of MontrealMontreal Quebec Canada H3C 3J7;</p> <p>Department of ChemistryUniversity of MontrealMontreal Quebec Canada H3C 3J7;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cholesterol&option=203" rel="nofollow">cholesterol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=imaging mass spectrometry&option=203" rel="nofollow">imaging mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=olefin&option=203" rel="nofollow">olefin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sample preparation&option=203" rel="nofollow">sample preparation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=silver nitrate&option=203" rel="nofollow">silver nitrate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spray deposition&option=203" rel="nofollow">spray deposition;</a> </p> <div class="translation"> 机译：胆固醇;成像质谱;烯烃;样品制备;银硝酸盐;喷雾沉积; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025632981.html">Silver spray deposition for AgLDI imaging MS of cholesterol and other olefins on thin tissue sections</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Ethan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Ethan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fournelle Frédéric&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Fournelle Frédéric,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chaurand Pierre&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chaurand Pierre </a> <a href="/journal-foreign-34906/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of mass spectrometry: JMS .</a> <span>2020</span><span>,第4期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于Agldi成像胆固醇和其他烯烃在薄组织部分上的银喷雾沉积</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704018313115.html">Silver-Assisted Laser Desorption Ionization For High Spatial Resolution Imaging Mass Spectrometry of Olefins from Thin Tissue Sections</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Martin Dufresne&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Martin Dufresne,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aurelien Thomas&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Aurelien Thomas,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Julien Breault-Turcot&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Julien Breault-Turcot,</a> <a href="/journal-foreign-19014/" target="_blank" rel="nofollow" class="tuijian_authcolor">Analytical chemistry .</a> <span>2013</span><span>,第6期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：银辅助激光解吸电离，用于薄组织切片中烯烃的高空间分辨率成像质谱分析</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070408126517.html">Matrix vapor deposition/recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ionization imaging mass spectrometry (SMALDI-MS) of tissue and single cells</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bouschen W&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bouschen W,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Schulz O&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Schulz O,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eikel D&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Eikel D,</a> <a href="/journal-foreign-26627/" target="_blank" rel="nofollow" class="tuijian_authcolor">Rapid Communications in Mass Spectrometry: RCM .</a> <span>2010</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于组织和单个细胞的高分辨率扫描微探针基质辅助激光解吸/电离成像质谱（SMALDI-MS）的基质气相沉积/重结晶和专用喷雾制备</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-257539_thesis/0705010940989.html">Silver assisted LDI for high spatial resolution imaging MS of olefins from thin tissue sections. 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