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Identification of spoilage by Alicyclobacillus bacteria in tomato‐based products by UHPLC‐MS/MS

机译：UHPLC-MS / MS鉴定甘氨酸中脂族产品的腐败物质

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Abstract > In recent years, there have been some cases of alteration of tomato derivatives by Alicyclobacilli spp. The spoilage was reported by consumers who felt odors and abnormal tastes (“bad”) of products. In this work, an untarget approach with Q‐Exactive? Quadrupole‐Orbitrap Mass Spectrometer has been used to identify metabolites associated with the alteration of tomato puree by Alicyclobacillus bacteria. The study was carried out on tomato puree found positive for the presence of Alicyclobacillus spores. The germination and growth of native spores were induced through incubation at 65°C under static conditions for 5?days. For microbial concentration below 100?ufc/mL, negative growth was considered. The samples arising from incubation were submitted to UHPL‐HRMS/MS analysis. To find compounds related to microbial growth, data obtained by UHPLC‐MS/MS analysis were processed with Thermo Fisher Compound Discoverer? 2.0 software. We compared samples grouped according to microbial growth: positive growth vs negative growth. Five compounds were identified and confirmed by analysis performed on authentic standards. To other compounds, we have assigned a putative identity. The HRMS approach using Q‐Exactive? Quadrupole‐Orbitrap Mass Spectrometer has proven to be an important and powerful tool for identification of compounds which could be used as marker molecules in cases of suspected spoilage by Alicyclobacillus bacteria in tomato derivatives. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”> xpractt </ title> >最近多年来，通过 alicyclobacilli </ i> SPP，已经存在一些番茄衍生物的案例。腐败的消费者报告了产品的异味和异常的产品（“坏”）。在这项工作中，Q-Facactive的无前方方法？ Quadrupole-orbitrap质谱仪已被用于鉴定与番茄丙酸酯的改变相关的代谢物，并且 alicyclobacillus bacteria。该研究在番茄酱对氨酸灭菌进行了阳性，用于血小菊属植物的存在阳性。通过在65℃下在静态条件下培养5？天，通过在65℃温育诱导天然孢子的萌发和生长。对于低于100〜UFC / ml的微生物浓度，考虑了负生长。将由孵育引起的样品提交给UHPL-HRMS / MS分析。为了发现与微生物生长有关的化合物，通过Thermo Fisher复合发现者处理了通过UHPLC-MS / MS分析获得的数据？ 2.0软件。我们将根据微生物生长进行比较分组的样品：阳性生长与负生长。通过对真实标准进行的分析确定并确认五种化合物。对于其他化合物，我们已经指定了一个推定的身份。使用Q-Facactive的HRMS方法？ Quadrupole-orbitrap质谱仪已被证明是一种重要而强大的工具，可用于鉴定在番茄衍生物中的 alicyclobacillus </ i>细菌的疑似腐败的病例中可用作标记分子的化合物。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第9期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cocconi Emanuela&option=202" target="_blank" rel="nofollow">Cocconi Emanuela;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Franceschini Barbara&option=202" target="_blank" rel="nofollow">Franceschini Barbara;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Previdi Maria Paola&option=202" target="_blank" rel="nofollow">Previdi Maria Paola;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Stazione Sperimentale per l'Industria delle Conserve Alimentari (SSICA)Parma Italy;</p> <p>Stazione Sperimentale per l'Industria delle Conserve Alimentari (SSICA)Parma Italy;</p> <p>Stazione Sperimentale per l'Industria delle Conserve Alimentari (SSICA)Parma Italy;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alicyclobacillus bacteria&option=203" rel="nofollow">Alicyclobacillus bacteria;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolites&option=203" rel="nofollow">metabolites;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Q‐Exactive&option=203" rel="nofollow">Q‐Exactive;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tomato puree&option=203" rel="nofollow">tomato puree;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=untarget approach&option=203" rel="nofollow">untarget approach;</a> </p> <div class="translation"> 机译：alicyclobacillus细菌;代谢物;q- actactive;番茄酱;未确定的方法; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025632992.html">Identification of spoilage by Alicyclobacillus bacteria in tomato‐based products by UHPLC‐MS/MS</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cocconi Emanuela&option=202" target="_blank" rel="nofollow" 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