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首页> 外文期刊>Journal of mass spectrometry: JMS >Comparison of the fragmentation behavior of DNA and LNA single strands and duplexes
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Comparison of the fragmentation behavior of DNA and LNA single strands and duplexes

机译：DNA和LNA单链和双链体碎片行为的比较

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摘要

DNA and locked nucleic acid (LNA) were characterized as single strands, as well as double stranded DNA-DNA duplexes and DNA-LNA hybrids using tandem mass spectrometry with collision-induced dissociation. Additionally, ion mobility spectrometry was carried out on selected species. Oligonucleotide duplexes of different sequences-bearing mismatch positions and abasic sites of complementary DNA 15-mers-were investigated to unravel general trends in their stability in the gas phase. Single-stranded LNA oligonucleotides were also investigated with respect to their gas phase behavior and fragmentation upon collision-induced dissociation. In contrast to the collision-induced dissociation of DNA, almost no base loss was observed for LNAs. Here, backbone cleavages were the dominant dissociation pathways. This finding was further underlined by the need for higher activation energies. Base losses from the LNA strand were also absent in fragmentation experiments of the investigated DNA-LNA hybrid duplexes. While DNA-DNA duplexes dissociated easily into single stranded fragments, the high stability of DNA-LNA hybrids resulted in predominant fragmentation of the DNA part rather than the LNA, while base losses were only observed from the DNA single strand of the hybrid.
机译：DNA和锁定的核酸（LNA）表征为单链，以及使用串联质谱法的双链DNA-DNA双链体和DNA -LNA杂交物，其具有抗冲诱导的解离。另外，在所选物种上进行离子迁移光谱法。研究了不同序列的不匹配位置和互补DNA 15-MERS的脱脂位点的寡核苷酸双链体 - 在气相中的稳定性中进行了抗大概的趋势。还研究了单链LNA寡核苷酸在碰撞诱导的解离时对其气相行为和碎裂进行研究。与DNA的碰撞诱导的解离相比，对于LNA几乎没有观察到碱基损失。在这里，骨干裂解是主要的解离途径。这种发现进一步强调了更高的激活能量的需求。在研究的DNA -LNA杂交双链体的碎片实验中也不存在来自LNA链的基础损失。虽然DNA-DNA双链体容易分解成单链片段，但DNA-LNA杂交物的高稳定性导致DNA部分而不是LNA的主要破碎，而仅从杂交的DNA单链观察到基础损失。

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