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首页> 外文期刊>Journal of mass spectrometry: JMS >Mass spectrometric evidence for the modification of small molecules in a cobalt-60-irradiated rodent diet
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Mass spectrometric evidence for the modification of small molecules in a cobalt-60-irradiated rodent diet

机译：钴-60辐照啮齿动物饮食中的小分子改性小分子的质谱证据

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The purpose of this study was to investigate the effect of radiation on the content of animal diet constituents using global metabolomics. Aqueous methanolic extracts of control and cobalt-60-irradiated Teklad 7001 diets were comprehensively analyzed using nano-liquid chromatography-MS/MS. Among the over 2000 ions revealed by XCMS followed by data preprocessing, 94 positive and 143 negative metabolite ions had greater than 1.5-fold changes and p-values < 0.01. Use of METABOANALYST statistical software demonstrated complete separation of the irradiated and non-radiated diets in unsupervised principal components analysis and supervised partial least squares discriminant analysis. Irradiation led to an increase in the content of phytochemicals such as glucosinolates and oxidized lipids in the diet. Twenty-eightmetabolites that were significantly changed in the irradiated samples were putatively identified at the level of molecular formulae by MS/MS.MS/MSALL analysis of chloroform-methanol extracts of the irradiated diet showed increased levels of a number of unique linoleic acid-derived branched fatty acid esters of hydroxy fatty acids. These data imply that gamma irradiation of animal diets causes chemical changes to dietary components, which in turn may influence the risk of mammary cancer. Copyright (C) 2017 John Wiley & Sons, Ltd.
机译：本研究的目的是探讨辐射对使用全球代谢组学的动物饮食成分含量的影响。使用纳米液相色谱 - MS / MS全面地综合分析对照和钴-60辐射的Teklad 7001饮食中的水性甲醇醇提取物。在XCMS揭示的超过2000离子中，随后数据预处理，94个阳性和143个阴性代谢物离子具有大于1.5倍的变化和P值<0.01。使用元成分的统计软件的使用证明了在无监督的主要成分分析中完全分离辐照和非辐射饮食分析和监督偏最小二乘判别分析。照射导致饮食中植物素和氧化脂质等植物化学物质的含量增加。通过MS / MS.MS / MSALL分析的MS / MSALL分析在辐照饮食的氯仿 - 甲醇提取物的水平下鉴定了辐照样品中显着改变的二十八个代谢物。显示出多种独特的亚油酸衍生的水平增加羟基脂肪酸的支化脂肪酸酯。这些数据意味着动物饮食的γ辐射导致化学变化对膳食成分，这又可能影响乳腺癌的风险。版权所有（c）2017 John Wiley＆Sons，Ltd。

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