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首页> 外文期刊>Journal of mass spectrometry: JMS >Incubation with Cu(II) and Zn(II) salts enhances MALDI‐TOF mass spectra of amyloid‐beta and α‐synuclein toward in vivo analysis
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Incubation with Cu(II) and Zn(II) salts enhances MALDI‐TOF mass spectra of amyloid‐beta and α‐synuclein toward in vivo analysis

机译：与Cu（II）和Zn（II）盐温育增强淀粉样蛋白-β和α-突触核蛋蛋白的MALDI-TOF质谱朝向体内分析

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Abstract >Insoluble senile plaque aggregates are indicative of Alzheimer's disease pathology. A similar phenomenon occurs in Parkinson's disease with the build‐up of Lewy bodies. The analysis of senile plaques, and other brain samples, from Alzheimer's disease and Parkinson's disease patients by matrix‐assisted laser desorption/ionization mass spectrometry has advantages but also presents obstacles because of the nature of the processes utilized in isolation procedures and storage. Salts, buffers, and detergents necessary in the isolation of biological species may cause adducts and ion suppression that convolute the spectra obtained. We previously determined that amyloid‐beta from isolated senile plaque deposits fragment similarly to the synthetic 40 and 42 amino acid peptide when analyzed by matrix‐assisted laser desorption/ionization mass spectrometry. In addition, α‐synuclein also fragments predictably by in‐source decay. This provides information that may be applied to the identification and localization of amyloid‐beta and α‐synuclein in senile plaques and intact tissue sections. Ion suppression must still be accounted for when analyzing biological samples, which makes identifying fragments at lower abundance difficult. The addition of certain transition‐metal salts (Cu(II), Zn(II)) to the sample prior to analysis serves to “clean” the spectra and allow the peptide fragments produced to be observed with a much higher signal to noise and occasionally, improved resolution. We present a systematic study of incubation with different metal salts and their impact on the quality of the spectra, as well as the role of the binding of the metals to the model biological compounds, obtained for synthetic amyloid‐beta, synthetic α‐synuclein, and isolated senile plaques. The optimized sample preparation methods presented will provide f </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >不溶性老年人斑块聚集体表示阿尔茨海默病病理学。在帕金森病的疾病中发生了类似的现象。乐骨体系。通过基质辅助激光解吸/电离质谱法从阿尔茨海默病和帕金森病患者的老年斑块和其他脑样本的分析具有优点，但由于分离程序和储存的过程的性质，因此也具有障碍。在分离生物物种中，必须引起加合物和离子抑制的盐，缓冲液和洗涤剂，其扭转所获得的光谱。当通过基质辅助激光解吸/电离质谱法分析时，我们以前确定了与合成40和42氨基酸肽类似的淀粉样蛋白β类似于合成40和42氨基酸肽。此外，α-突触核蛋白也通过源极衰减可预测的片段。这提供了可以应用于老年斑块和完整组织切片中淀粉样蛋白β和α-突触核蛋白的鉴定和定位的信息。在分析生物样品时仍然仍然难以考虑离子抑制，这使得在较低的丰度下识别碎片。在分析之前，在样品中添加某些过渡金属盐（Cu（II），Zn（II））用于“清洁”光谱，并允许制备的肽片段以更高的信号噪音和偶尔观察到捕获的肽片段，改进了分辨率。我们对不同的金属盐孵育及其对光谱质量的影响，以及金属与合成淀粉样蛋白 - β，合成α-突触核蛋白的基础生物化合物的作用以及合成α-突触核蛋白的作用的系统研究。和孤立的老年斑块。提供的优化样品制备方法将提供f </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第2期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kelley Andrea R.&option=202" target="_blank" rel="nofollow">Kelley Andrea R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Colley Madeline E.&option=202" target="_blank" rel="nofollow">Colley Madeline E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Perry George&option=202" target="_blank" rel="nofollow">Perry George;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bach Stephan B.H.&option=202" target="_blank" rel="nofollow">Bach Stephan B.H.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryUniversity of Texas at San AntonioOne UTSA Blvd. San Antonio TX USA;</p> <p>Department of ChemistryUniversity of Texas at San AntonioOne UTSA Blvd. San Antonio TX USA;</p> <p>Department of ChemistryUniversity of Texas at San AntonioOne UTSA Blvd. San Antonio TX USA;</p> <p>Department of ChemistryUniversity of Texas at San AntonioOne UTSA Blvd. San Antonio TX USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=a‐synuclein&option=203" rel="nofollow">a‐synuclein;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alzheimer's disease&option=203" rel="nofollow">Alzheimer's disease;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=amyloid‐beta&option=203" rel="nofollow">amyloid‐beta;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI&option=203" rel="nofollow">MALDI;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Parkinson's disease&option=203" rel="nofollow">Parkinson's disease;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sample preparation&option=203" rel="nofollow">sample preparation;</a> </p> <div class="translation"> 机译：A-突触核蛋白;阿尔茨海默病;淀粉样蛋白β;马尔迪;质谱;帕金森病;样品制备; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘涛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘涛</a> <span> <a href="/journal-cn-56701/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国生物工程杂志 </a> </span> <span> . 2007</span><span>,第4期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130466265168.html">METHOD OF METALLIC IONES CU(II),PB(II),HG(II),CD(II),NI(II),ZN(II),SR(II),FE(III),LA(III), UO2 2+,TH(IV) REMOVAL FROM AQUEOUS SOLUTIONS</a> <b>[P]</b> . <span> 外国专利： <!-- --> CS270845B1 </span> <span> . 1990-08-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：金属离子的方法铜（II），铅（II），HG（II），CD（II），NI（II），ZN（II），SR（II），FE（III），LA（III），UO2从水溶液中去除2 +，TH（IV） </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130466258567.html">METHOD OF METALLIC IONES CU(II),PB(II),HG(II),CD(II),NI(II),ZN(II),SR(II),FE(III),LA(III), UO2 2+,TH(IV) REMOVAL FROM AQUEOUS SOLUTIONS</a> <b>[P]</b> . <span> 外国专利： <!-- --> CS8805771A1 </span> <span> . 1989-12-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：金属离子的方法铜（II），铅（II），HG（II），CD（II），NI（II），ZN（II），SR（II），FE（III），LA（III），UO2从水溶液中去除2 +，TH（IV） </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130466262807.html">METHOD OF METALLIC IONES CU(II),PB(II),HG(II),CD(II),NI(II),ZN(II),SR(II),FE(III),LA(III), UO2 2+,TH(IV) REMOVAL FROM AQUEOUS SOLUTIONS</a> <b>[P]</b> . <span> 外国专利： <!-- --> CS577188A1 </span> <span> . 1989-12-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：金属离子的方法铜（II），铅（II），HG（II），CD（II），NI（II），ZN（II），SR（II），FE（III），LA（III），UO2从水溶液中去除2 +，TH（IV） </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025633306','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p 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