首页> 外文期刊>Journal of mass spectrometry: JMS >Detergent‐assisted sample preparation for MALDI‐MS: Investigation of octylglucoside and docecylmaltoside for matrix crystallization, on‐plate digestion, and trypsin activity

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Detergent‐assisted sample preparation for MALDI‐MS: Investigation of octylglucoside and docecylmaltoside for matrix crystallization, on‐plate digestion, and trypsin activity

机译：用于MALDI-MS的洗涤剂辅助样品制剂：对基质结晶，板材消化和胰蛋白酶活性的辛基葡糖苷和多癸基霉菌的研究

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Abstract >We show an easy and fast method for improved detection of lipophilic peptides with MALDI‐MS utilizing the nonionic detergents n‐octylglucoside and n‐dodecylmaltoside (laurylmaltoside). Investigations comprised on‐plate digestion of proteins with trypsin, detergent effects on the protease trypsin, and the changes in MALDI matrix crystallization. Investigations also exhibited a higher tryptic activity in trypsin activity assay of 139% when using laurylmaltoside as supplement. Crystallization changed toward a more homogeneous crystal distribution and especially trypsinized insulin spectra recorded with MALDI‐MS showed improved detectability of lipophilic peptides. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >我们显示利用非离子洗涤剂N-辛基葡糖苷和N-十二烷基氨基甲醚（月桂酸酯）改善了利用非离子洗涤剂的MALDI-MS检测的简单且快速的方法。调查包括用胰蛋白酶，对蛋白酶胰蛋白酶的洗涤剂作用以及MALDI基质结晶的变化的调查。当使用月桂渣作为补充剂时，研究在139％的胰蛋白酶活性测定中也表现出更高的胰蛋白酶活性。结晶朝向更均匀的晶体分布变为更均匀的晶体分布，特别是用MALDI-MS记录的胰蛋白酶精化胰岛素光谱显示出改善亲脂肽的可检测性。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rühl Michael&option=202" target="_blank" rel="nofollow">Rühl Michael;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sch?nborn Stefan&option=202" target="_blank" rel="nofollow">Sch?nborn Stefan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karas Michael&option=202" target="_blank" rel="nofollow">Karas Michael;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Pharmaceutical Chemistry Goethe University FrankfurtMax‐von‐Laue‐Stra?e 9 D‐60438 Frankfurt Germany;</p> <p>Institute of Pharmaceutical Chemistry Goethe University FrankfurtMax‐von‐Laue‐Stra?e 9 D‐60438 Frankfurt Germany;</p> <p>Institute of Pharmaceutical Chemistry Goethe University FrankfurtMax‐von‐Laue‐Stra?e 9 D‐60438 Frankfurt Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipophilic peptides&option=203" rel="nofollow">lipophilic peptides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI‐MS&option=203" rel="nofollow">MALDI‐MS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=n‐laurylmaltoside&option=203" rel="nofollow">n‐laurylmaltoside;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=n‐octylglucoside&option=203" rel="nofollow">n‐octylglucoside;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nonionic detergents&option=203" rel="nofollow">nonionic detergents;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=on‐plate digestion&option=203" rel="nofollow">on‐plate digestion;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=trypsin activity&option=203" rel="nofollow">trypsin activity;</a> </p> <div class="translation"> 机译：亲脂肽;马尔迪-SMS;N-月桂基甲糖苷;正辛基糖苷;非离子洗涤剂;板材消化;胰蛋白酶活动; 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initial;overflow: initial;"> <span>机译：矩阵辅助激光解吸和电离（MALDI）样品板可释放地耦合到样品板适配器 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025633390','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: 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