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Combining MALDI-2 and transmission geometry laser optics to achieve high sensitivity for ultra-high spatial resolution surface analysis

机译:结合MALDI-2和传动几何激光光学器件,实现超高空间分辨率表面分析的高灵敏度

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摘要

A transmission geometry optical configuration allows for smaller laser spot size to facilitate high-resolution matrix-assisted laser/desorption ionization (MALDI) mass spectrometry. This increase in spatial resolution (ie, smaller laser spot size) is often associated with a decrease in analyte signal. MALDI-2 is a post-ionization technique, which irradiates ions and neutrals generated in the initial MALDI plume with a second orthogonal laser pulse, and has been shown to improve sensitivity. Herein, we have modified a commercial Orbitrap mass spectrometer to incorporate a transmission geometry MALDI source with MALDI-2 capabilities to improve sensitivity at higher spatial resolutions.
机译:传动几何光学配置允许较小的激光光斑尺寸,以促进高分辨率矩阵辅助激光/解吸电离(MALDI)质谱法。 这种空间分辨率(即,较小的激光光斑尺寸)的增加通常与分析物信号的降低相关。 MALDI-2是一种后电离技术,其用第二正交激光脉冲照射在初始MALDI羽流中产生的离子和中性,并且已经显示出提高灵敏度。 在此,我们已经修改了商业轨道物质光谱仪以将透射几何MALDI源与MALDI-2能力合并,以提高较高空间分辨率的灵敏度。

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