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首页> 外文期刊>Biotechnology Progress >Measurement of Cooling and Warming Rates in Vitrification-Based Plant Cryopreservation Protocols
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Measurement of Cooling and Warming Rates in Vitrification-Based Plant Cryopreservation Protocols

机译：基于玻璃化的植物冷冻保存规程中降温和升温速率的测量

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Cryopreservation protocols include the use of additives and pretreatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (T_f) and the glass transition (T_G) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between —20°C and —120°C, ranged from ca. 5°C s~(-1) to 400°C s~(-1), while warming rates spanned from ca. 2°C s~(-1) to 280°C s~(-1), for the different protocols and conditions studied. A wider measuring window (0°C to —150°C) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.
机译：冷冻保存方案包括使用添加剂和预处理，主要是通过微粘度增加来降低所有温度下冰核的可能性。仍然有在平衡冻结（T_f）和玻璃化转变（T_G）温度之间的温度区域内结冰的风险。因此，必须快速冷却和升温，尤其是在该区域，以避免冰源破坏。对玻璃化和液滴玻璃化技术（基于快速冷却的常用冷冻保存方案）以及相应的加热程序进行了研究。使用了一个非常快速的数据采集系统，该系统能够读取非常低的温度，低至液氮的温度。测得的冷却速度介于-20°C至-120°C之间，范围从大约。 5°C s〜（-1）至400°C s〜（-1），而升温速率约为针对所研究的不同方案和条件，温度范围为2°C s〜（-1）至280°C s〜（-1）。在所有情况下，较宽的测量窗口（0°C至-150°C）产生的速率较低。降温和升温速率也与不同程序后观察到的存活率有关。那些具有更快速率的方案产生了最高的存活百分比。

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