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首页> 外文期刊>Biotechnology Progress >Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance
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Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance

机译：用紫外线辐射对不定因子进行处理的细胞培养基：对CHO性能的影响最小

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摘要

Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation is a sterilization method effective against bacteria and viruses while being non-thermal and non-adulterating in its mechanism of action. This makes UV irradiation attractive for use in sterilization of cell culture media. The objective of this study was to evaluate the effect of UV irradiation of cell culture media in terms of chemical composition and the ability to grow cell cultures in the treated media. The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. The cumulative effect of these changes, however, did not negatively influence the ability to culture Chinese Hamster Ovary cells, as evaluated by cell viability, growth rate, and protein titer measurements in simple batch growth compared with the same cells cultured in control media exposed to visible light.
机译：细胞培养基的无菌性是生物治疗过程中的重要问题。在大规模的生物治疗生产中，细胞培养基的单位污染会产生昂贵的影响。紫外线（UV）辐照是一种对细菌和病毒有效的杀菌方法，同时在作用机理上不发热且不受污染。这使得紫外线辐射可用于细胞培养基的灭菌。这项研究的目的是根据化学成分和在处理过的培养基中生长细胞培养物的能力来评估细胞培养基的紫外线照射的效果。结果表明，商业化细胞培养基以相应的消毒剂量进行紫外线照射会影响培养基中相对于几种羧酸的化学组成，并且在最小程度上会影响氨基酸。然而，这些变化的累积效应并未对培养中国仓鼠卵巢细胞的能力产生负面影响，与暴露于暴露于对照培养基中的相同细胞相比，通过简单的分批生长中的细胞活力，生长速率和蛋白滴度测量可以评估这些能力可见光。

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