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首页> 外文期刊>Biotechnology Progress >Characterization of the cellulolytic secretome of Trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin
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Characterization of the cellulolytic secretome of Trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin

机译：哈茨木霉在甘蔗渣上生长过程中的纤维素分解分泌物的表征以及膨胀素活性增强作用的分析

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This study demonstrates the production of an active enzyme cocktail produced by growing Trichoderma harzianum on sugarcane bagasse. The component enzymes were identified by LCMS-MS. Glycosyl hydrolases were the most abundant class of proteins, representing 67% of total secreted protein. Other carbohydrate active enzymes involved in cell wall deconstruction included lytic polysaccharide mono-oxygenases (AA9), carbohydrate-binding modules, carbohydrate esterases and swollenin, all present at levels of 1%. In total, proteases and lipases represented 5 and 1% of the total secretome, respectively, with the rest of the secretome being made up of proteins of unknown or putative function. This enzyme cocktail was efficient in catalysing the hydrolysis of sugarcane bagasse cellulolignin to fermentable sugars for potential use in ethanol production. Apart from mapping the secretome of T. harzianum, which is a very important tool to understand the catalytic performance of enzyme cocktails, the gene coding for T. harzianum swollenin was expressed in Aspergillus niger. This novel aspect in this work, allowed increasing the swollenin concentration by 95 fold. This is the first report about the heterologous expression of swollenin from T. harzianum, and the findings are of interest in enriching enzyme cocktail with this important accessory protein which takes part in the cellulose amorphogenesis. Despite lacking detectable glycoside activity, the addition of swollenin of T. harzianum increased by two-fold the hydrolysis efficiency of a commercial cellulase cocktail. (c) 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:327-336, 2016
机译：这项研究证明了由哈茨木霉在甘蔗渣上生长产生的活性酶混合物的产生。通过LCMS-MS鉴定组分酶。糖基水解酶是最丰富的蛋白质类别，占总分泌蛋白质的67％。参与细胞壁解构的其他碳水化合物活性酶包括裂解多糖单加氧酶（AA9），碳水化合物结合模块，碳水化合物酯酶和膨胀素，它们的含量均为1％。总的来说，蛋白酶和脂肪酶分别占总分泌蛋白的5％和1％，其余的分泌蛋白由未知或假定功能的蛋白质组成。该酶混合物有效催化甘蔗渣纤维素纤维素素水解成可发酵糖，可用于乙醇生产。除了绘制哈茨木霉的分泌蛋白图谱（这是了解酶混合物催化性能的非常重要的工具）之外，黑曲霉还表达了编码哈茨木霉swollenin的基因。这项工作中的这一新颖方面使溶胀素浓度增加了95倍。这是关于哈茨木霉中swollenin异源表达的第一个报道，这一发现对于利用参与纤维素无定形形成的这一重要辅助蛋白富集酶混合物是有意义的。尽管缺乏可检测的糖苷活性，但是哈茨木霉的swollenin的添加使商业纤维素酶混合物的水解效率提高了两倍。 （c）2016美国化学工程师学会生物技术学会。 Prog。，32：327-336，2016

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