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Effect of Aeration and Agitation on Extractive Fermentation of Clavulanic Acid by Using Aqueous Two-phase System

机译：两相水曝气和搅拌对克拉维酸提取发酵的影响

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In this work, the effects of agitation and aeration rates on aqueous two-phase system (ATPS)-based extractive fermentation of clavulanic acid (CA) by Streptomyces variabilis DAUFPE 3060 were investigated through a 2(2) full factorial design, where oxygen transfer rate (OTR) and oxygen uptake rate (OUR) were selected as the responses. Aeration rates significantly influenced cell growth, OUR, and CA yield, while OTR was practically the same in all the runs. Under the intermediate agitation (950 rpm) and aeration conditions (3.5 vvm) of the central point runs, it was achieved OTR of 1.617 +/- 0.049 mmol L-1 h(-1), OUR of 0.132 +/- 0.030 mmol L-1 h(-1), maximum CA production of 434 +/- 4 mg L-1, oxygen mass transfer coefficient of 33.40 +/- 2.01 s(-1), partition coefficient of 66.5 +/- 1.5, CA yield in the top and bottom phases of 75% +/- 2% and 19% +/- 1%, respectively, mass balance of 95% +/- 4% and purification factor of 3.8 +/- 0.1. These results not only confirmed the paramount role of O-2 supply, broth composition and operational conditions in CA ATPS-extractive fermentation, but also demonstrated the possibility of effectively using this technology as a cheap tool to simultaneously produce and recover CA. (C) 2016 American Institute of Chemical Engineers
机译：在这项工作中，通过变因子链霉菌DAUFPE 3060研究了搅拌和曝气速率对基于水两相系统（ATPS）的克拉维酸（CA）萃取发酵的影响，该设计通过2（2）全因子设计进行，其中氧转移反应速率（OTR）和摄氧率（OUR）被选择。曝气速率显着影响细胞生长，OUR和CA产量，而OTR在所有运行中几乎相同。在中心点运行的中间搅拌（950 rpm）和通气条件（3.5 vvm）下，OTR为1.617 +/- 0.049 mmol L-1 h（-1），OUR为0.132 +/- 0.030 mmol L -1 h（-1），最大CA产量为434 +/- 4 mg L-1，氧气传质系数为33.40 +/- 2.01 s（-1），分配系数为66.5 +/- 1.5，CA收率顶部和底部相分别为75％+/- 2％和19％+/- 1％，质量平衡为95％+/- 4％，纯化系数为3.8 +/- 0.1。这些结果不仅证实了O-2供应，肉汤成分和操作条件在CA ATPS提取发酵中的最重要作用，而且还证明了有效利用该技术作为同时生产和回收CA的廉价工具的可能性。 （C）2016美国化学工程师学会

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