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Combined Effect of Hydrodynamic and Interfacial Flow Parameters on Lysozyme Deactivation in a Stirred Tank Bioreactor

机译:流体动力学和界面流动参数对搅拌罐生物反应器中溶菌酶失活的综合影响

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The dynamic environment within a bioreactor and in the purification equipment is known to affect the activity and yield of enzyme production.The present research focuses on the effect of hydrodynamic flow parameters (average energy dissipation rate,maximum energy dissipation rate,average shear rate,and average normal stress)and the interfacial flow parameters (specific interfacial area and mass transfer coefficient)on the activity of lysozyme.Flow parameters were estimated using CFD simulation based on the k-epsilon approch.Enzyme deactivation was investigated in 0.1,0.3,0.57,and 1 m i.d.vessels.Enzyme solution was subjected to hydrodynamic stress using various types of impellers and impeller combinations over a wide range of power consumption (0.03
机译:众所周知,生物反应器内部和纯化设备中的动态环境会影响酶的生产活性和产量。本研究的重点是流体动力流参数(平均能量耗散率,最大能量耗散率,平均剪切率和平均溶应力和界面流动参数(比界面面积和传质系数)对溶菌酶活性的影响。基于k-ε方法的CFD模拟估算了流动参数。在0.1、0.3、0.57中研究了酶的失活,酶溶液通过各种类型的叶轮和叶轮组合在很宽的功率消耗范围内(0.03

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