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The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

机译:使用PCR和BSMAI限制组合靶向WCIP基因以确定血清型6a,6b,6c和6d链球菌肺炎料

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摘要

We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.
机译:我们设计了一种敏感且可靠的方法,以利用PCR来区分血清群6,然后用酶限制性消化。 我们发现,该血清型化方法能够基于WCIP区域中BSMAI的识别位点来区分血清型6a,6b,6c和6d。

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