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首页> 外文期刊>Biotechnology Progress >Co super(2+), Cu super(2+), and Zn super(2+) Accumulation byCyanobacterium Spirulina platensis
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Co super(2+), Cu super(2+), and Zn super(2+) Accumulation byCyanobacterium Spirulina platensis

机译：蓝藻螺旋藻对钴超（2+），铜超（2+）和锌超（2+）的积累

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The Spirulina platensis biomass was characterized for its metal accumulation as a function of pH, external metal concentration, equilibrium isotherms, kinetics, effect of co-ions under free (living cells, lyophilized, and oven-dried) and immobilized (Ca-alginate and polyacrylamide gel) conditions. The maximum metal biosorption by S. platensis biomass was observed at pH 6.0 with free and immobilized biomass. The studies on equilibrium isotherm experiments showed highest maximum metal loading by living cells (181.0 plus or minus 13.1 mg Co super(2+)/g, 272.1 plus or minus 29.4 mg Cu super(2+)/g and 250.3 plus or minus 26.4 mg Zn super(2+)/g) followed by lyophilized (79.7 plus or minus 9.6 mg Co super(2+)/g, 250.0 plus or minus 22.4 mg Cu super(2+)/g and 111.2 plus or minus 9.8 mg Zn super(2+)/g) and oven-dried (25.9 plus or minus 1.9 mg Co super(2+)/g, 160.0 plus or minus 14.2 mg Cu super(2+)/g and 35.1 plus or minus 2.7 mg Zn super(2+)/g) biomass of S. platensis on a dry weight basis. The polyacrylamide gel (PAG) immobilization of lyophilized biomass found to be superior over Ca-alginate (Ca-Alg) and did not interfere with the S. platensis biomass biosorption capacity, yielding 25% of metal loading after PAG entrapment. The time-dependent metal biosorption in both the free and immobilized form revealed existence of two phases involving an initial rapid phase (which lasted for 1--2 min) contributing 63--77% of total biosorption, followed by a slower phase that continued for 2 h. The metal elution studies conducted using various reagents showed more than 90% elution with mineral acids, calcium salts, and Na sub(2)EDTA with free (lyophilized or oven-dried) as well as immobilized biomass. The experiments conducted to examine the suitability of PAG-immobilized S. platensis biomass over multiple cycles of Co super(2+), Cu super(2+), and Zn super(2+) sorption and elution showed that the same PAG cubes can be reused for at least seven cycles with high efficiency.
机译：螺旋藻生物量的特征在于其金属积累随pH，外部金属浓度，平衡等温线，动力学，游离离子（活细胞，冻干和烘箱干燥）和固定化（钙藻酸盐和聚丙烯酰胺凝胶）条件。在游离和固定化的生物量下，pH 6.0观察到S. platensis生物量对金属的最大生物吸附。平衡等温线实验的研究表明，活细胞的最大金属负载量最高（181.0正负13.1 mg钴超（2 +）/ g，272.1正负29.4 mg铜超（2 +）/ g和250.3正负26.4。毫克锌超级（2 +）/ g），然后冻干（79.7正负9.6毫克钴超级（2 +）/ g，250.0正负22.4毫克铜超级（2 +）/ g和111.2正负9.8毫克锌超级（2 +）/ g）和烘干（25.9正负1.9 mg钴超级（2 +）/ g，160.0正负14.2 mg铜超级（2 +）/ g和35.1正负2.7 mg基于干重的S. platensis Zn super（2 +）/ g）生物量。冻干生物质的聚丙烯酰胺凝胶（PAG）固定化效果优于藻藻酸钙（Ca-Alg），并且不干扰平板酵母生物量的生物吸收能力，在捕获PAG后产生25％的金属负载量。游离和固定形式下随时间变化的金属生物吸附表明存在两个阶段，涉及初始快速阶段（持续1-2分钟），占总生物吸附的63--77％，随后是缓慢阶段，继续2小时。使用各种试剂进行的金属洗脱研究表明，游离酸（冻干或烘箱干燥）以及固定化的生物质能用无机酸，钙盐和Na sub（2）EDTA洗脱90％以上。通过实验研究了PAG固定化的S. platensis生物量在Co super（2 +），Cu super（2+）和Zn super（2+）吸附和洗脱的多个循环中的适用性，结果表明相同的PAG立方体可以至少可以高效重复使用七个周期。

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