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首页> 外文期刊>Journal of microbiology and biotechnology >Complete Genome of Bacillus subtilis subsp subtilis KCTC 3135(T) and Variation in Cell Wail genes of B. subtilis Strains
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Complete Genome of Bacillus subtilis subsp subtilis KCTC 3135(T) and Variation in Cell Wail genes of B. subtilis Strains

机译：Bacillus subtilis subtilis凋亡菌Kctc 3135（t）的完整基因组，以及B.枯草芽孢杆菌菌株的细胞致癌基因的变异

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The type strain Bacillus subtilis subsp. subtilis KCTC 3135(T) was deeply sequenced and annotated, replacing a previous draft genome in this study. The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii. However, a comparative genomic analysis of B. subtilis spp. revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls. These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis. The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation. The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies. These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.
机译：菌株芽孢杆菌枯草芽孢杆菌子株。枯草芽孢杆菌KCTC 3135（T）深入测序和注释，替换本研究中的先前的基因组。焦油和标签基因涉及合成壁吡辛酸（WTA），并且先前认为这些基因及其产物被认为是B.S之间的显着差异。枯草芽孢杆菌和B. s。 spizizenii。然而，B.枯草芽孢杆菌SPP的比较基因组分析。透露B. s。枯草芽孢杆菌和B. s。 Spizizenii有各种类型的细胞壁。这些焦油和标签操纵子是相互排斥的，B. s的焦油基因。与来自B.S的标签基因不同，Spizizenii与来自非Bacillus细菌的基因非常相似。枯草芽孢杆菌。结果和先前的研究表明，Tar基因和标签基因在亚种类形态之后不遗传。基于全基因组序列的系统发育树表明，每个亚种清楚地形成一组单位基团，而基于焦油基因的树表明单晶基团是根据细胞壁类型而不是亚种形成的。这些发现表明，作为细胞壁组分的焦油基因和基质醇的存在不是B.枯草芽孢杆菌的亚种与亚种B.S的描述之间的显着差异。应该更新spizizenii。

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