• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Analysis of the Statistical Thermodynamic Model for Nonlinear Binary Protein Adsorption Equilibria
【24h】

Analysis of the Statistical Thermodynamic Model for Nonlinear Binary Protein Adsorption Equilibria

机译：非线性二元蛋白质吸附平衡的统计热力学模型分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The statistical thermodynamic(ST)model was used to study nonlinear binary protein adsorption equilibria on an anion exchanger.Single-component and binary protein adsorption isotherms of bovine hemoglobin(Hb)and bovine serum albumin(BSA)on DEAE Spherodex M were determined by batch adsorption experiments in 10 mM Tris-HCl buffer containing a specific NaCl concentration(0.05,0.10,and 0.15 M)at pH 7.40.The ST model was found to depict the effect of ionic strength on the single-component equilibria well,with model parameters depending on ionic strength.Moreover,the ST model gave acceptable fitting to the binary adsorption data with the fitted single-component model parameters,leading to the estimation of the binary ST model parameter.The effects of ionic strength on the model parameters are reasonably interpreted by the electrostatic and thermodynamic theories.The effective charge of protein in adsorption phase can be separately calculated from the two categories of the model parameters,and the values obtained from the two methods are consistent.The results demonstrate the utility of the ST model for describing nonlinear binary protein adsorption equilibria.
机译：用统计热力学（ST）模型研究阴离子交换剂上非线性二元蛋白质的吸附平衡。分批测定牛血红蛋白（Hb）和牛血清白蛋白（BSA）在DEAE Spherodex M上的单组分和二元蛋白质吸附等温线。在pH值为7.40的特定NaCl浓度（0.05、0.10和0.15 M）的10 mM Tris-HCl缓冲液中进行吸附实验。通过模型参数发现ST模型可以很好地描述离子强度对单组分平衡的影响此外，ST模型通过拟合的单组分模型参数对二元吸附数据进行了可接受的拟合，从而估计了二元ST模型参数。合理解释了离子强度对模型参数的影响可以通过两类模型参数分别计算出吸附相中蛋白质的有效电荷，结果表明，ST模型可用于描述非线性二元蛋白质吸附平衡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号