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首页> 外文期刊>Journal of Medical Virology >Genetic characterization of enterovirus 71 isolated from patients with severe disease by comparative analysis of complete genomes
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Genetic characterization of enterovirus 71 isolated from patients with severe disease by comparative analysis of complete genomes

机译：通过完全基因组对比分析肠病病毒71肠道病毒71分离的遗传表征

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摘要

Enterovirus 71 (EV71) which causes mild illness in children is also associated with severe neurological complications. This study analyzed the complete genomes of EV71 strains derived from mild and severe diseases in order to determine whether the differences of EV71 genomes were responsible for different clinical presentations. Compared to complete genomes of EV71 strains derived from mild cases (less virulent strains), nucleotide differences in EV71 strains isolated from severe cases (more virulent strains) were observed primarily in the internal ribosomal entry site (IRES) of the 5′-untranslated region (UTR), which is vital for the cap-independent translation of viral proteins. In the protein-coding region, an E-Q substitution at amino acid position 145 of structural protein VP1 that occurred in more than one of more virulent strains was observed. This site is known to be related functionally to receptor binding and virulence in mice. Overall, strains (Group III) isolated from patients with fatal or severe sequelae outcomes had greater sequence substitutions in the 5′-UTR and/or protein-coding region and exhibited a relatively low-average homology to less virulent strains across the entire genome, indicating the possibility of significant genomic diversity in the most virulent EV71 strains. Further studies of EV71 pathogenesis should examine the significance of genomic diversity and the effects of multiple mutations in a viral population.
机译：导致儿童轻度疾病的肠道病毒71（EV71）也与严重的神经系统相关有关。本研究分析了来自轻度和严重疾病的EV71菌株的完整基因组，以确定EV71基因组的差异是否对不同的临床介绍负责。与来自轻度病​​例（较小的毒性菌株）的EV71菌株的完全基因组相比，从严重病例（更高毒性菌株）中分离的EV71菌株中的核苷酸差异主要在5' - 未转换区域的内部核糖体进入部位（IRE）中观察到（UTR），这对于无关的病毒蛋白的概括翻译至关重要。在蛋白质编码区域中，观察到在多于一种更毒性菌株中发生的结构蛋白VP1的结构蛋白VP1的氨基酸位置145的E-Q取代。已知该网站与小鼠的受体结合和毒力有关。总体而言，从致命或严重的后遗症结果中分离的菌株（III组）在5'--UTR和/或蛋白质编码区中具有更大的序列取代，并且在整个基因组上表现出相对低于毒性的毒株的同源性，表明最具毒性EV71菌株中显着基因组多样性的可能性。 EV71发病机制的进一步研究应研究基因组多样性的重要性和病毒群体中多种突变的作用。

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  • 来源
    《Journal of Medical Virology》 |2012年第6期|共9页
  • 作者

    ChangS.-C.; LiW.-C.; ChenG.-W.; TsaoK.-C.; HuangC.-G.; HuangY.-C.; ChiuC.-H.; KuoC.-Y.; TsaiK.-N.; ShihS.-R.; LinT.-Y.;

  • 作者单位

    Research Center for Emerging Viral Infections Chang Gung University Taoyuan Taiwan;

    Division of Infectious Diseases Department of Pediatrics Chang Gung Children's Hospital Chang;

    Research Center for Emerging Viral Infections Chang Gung University Taoyuan Taiwan Department;

    Clinical Virology Laboratory Department of Laboratory Medicine Chang Gung Memorial Hospital;

    Clinical Virology Laboratory Department of Laboratory Medicine Chang Gung Memorial Hospital;

    Division of Infectious Diseases Department of Pediatrics Chang Gung Children's Hospital Chang;

    Division of Infectious Diseases Department of Pediatrics Chang Gung Children's Hospital Chang;

    Division of Infectious Diseases Department of Pediatrics Chang Gung Children's Hospital Chang;

    Research Center for Emerging Viral Infections Chang Gung University Taoyuan Taiwan;

    Research Center for Emerging Viral Infections Chang Gung University Taoyuan Taiwan Clinical;

    Research Center for Emerging Viral Infections Chang Gung University Taoyuan Taiwan Division of;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学微生物学（病原细菌学、病原微生物学）;
  • 关键词

    Diversity; EV71; IRES; Sequence; VP1-145;

    机译：多样性;EV71;IRES;序列;VP1-145;

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