...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Virology >Detection and quantification of Merkel cell polyomavirus. Analysis of Merkel cell carcinoma cases from 1977 to 2015
【24h】

Detection and quantification of Merkel cell polyomavirus. Analysis of Merkel cell carcinoma cases from 1977 to 2015

机译：梅尔克尔细胞多马病毒的检测和定量。 1977年至2015年梅尔克尔细胞癌病例分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigates the presence of Merkel cell polyomavirus (MCPyV) in skin lesions of patients with Merkel cell carcinoma (MCC). MCPyV was quantified using quantitative Real-Time-PCR (qRT-PCR) in 34 paraffinized MCC samples (resected/biopsied) originally taken between 1977 and 2015, and six non-MCC samples. In 31 (91.2%) MCC-individuals, MCPyV was detected. No virus was observed in any non-MCC tumor. Average age at diagnosis was 78.2 +/- 9.35 (55-97) years for women (n = 19) and 69.5 +/- 14.7 (45-91) for men (n = 15) (P = 0.04). MCC tumor location, known in 25 cases, was: 11 (44%) in the head region, 6 (24%) in upper limbs, 4 (16%) in lower limbs, and 4 (16%) in the trunk. All but one patient had received some sort of treatment: 15 (45.45%) underwent both radio and chemotherapy, 13 (39.39%) only surgery, 2 (6.06%) surgery, plus radio and chemotherapy, 2 (6.06%) surgery and chemotherapy, and 1 (3.03%) only radiotherapy. Follow up data were available for 21/34 patients: recurrence was recorded for 4 (19.04%), and metastasis for 13 (61.9%). Recorded data showed that 10 men and 5 women (total 44.1%) died during follow up, 7 (46.7%) of them within 2 years of diagnosis. Viral load was 5.8 +/- 1.4 log copies/10(5) cells (3.1-8.6), independent of any variable. MCPyV was very frequent in MCC. It was principally associated with head and limb tumors, it more commonly affected men, who in this study were, on average, younger than women, and had high rates of recurrence and mortality. The amplification techniques described here are easily applied and suitable for detecting the presence of MCPyV virus in MCC.
机译：本研究研究了Merkel细胞癌（MCC）患者皮肤病变中Merkel CelloMavirus（MCPyV）的存在。在1977年至2015年之间的34个中途化的MCC样品（切除/活检）中，使用定量实时-PCR（QRT-PCR）量化了McPyV。在31中（91.2％）MCC-yands，检测到McPyV。在任何非MCC肿瘤中没有观察到病毒。诊断的平均年龄为78.2 +/- 9.35（55-97）岁（n = 19）和男性的69.5 +/- 14.7（45-91）（n = 15）（p = 0.04）。 MCC肿瘤位置，在25例中已知是：11（44％）在头部区域，6（24％）中肢，下肢4（16％），树干中的4（16％）。除了一名患者已经接受了某种治疗方法：15（45.45％）接受无线电和化疗，13（39.39％）仅手术，2（6.06％）手术，加上广播和化疗，2（6.06％）手术和化疗和1（3.03％）仅放射疗法。适用于21/34患者的随访数据：记录4（19.04％）的复发，并转移为13（61.9％）。记录的数据显示，在诊断2年内，10名男性和5名女性（共44.1％）在后续，7（46.7％）。病毒载荷为5.8 +/- 1.4日志副本/ 10（5）个单元（3.1-8.6），与任何变量无关。 MCCV在MCC中非常频繁。它主要与头部和肢体肿瘤有关，它更常见的男性，在这项研究中，平均而言，比女性更年轻，并且具有高的复发和死亡率。这里描述的扩增技术易于应用并且适合于检测MCC中的McPyV病毒的存在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号