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Design and Construction of a Preparative-Scale Dynamic Field Gradient Focusing Apparatus

机译:制备规模的动态场梯度聚焦装置的设计与构建

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A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, ~O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.
机译:使用线性模型显示动态场梯度聚焦(DFGF)可以缩放至制备能力〜O(10毫克)。本文介绍了如何设计和制造制备规模的DFGF设备。按比例缩小的实验和数学建模指导了构造过程中材料的选择和设计变更,以增加原型制备规模DFGF仪器将按预期执行的可能性。最终的原型成功地将牛血红蛋白从初始浓度6.82集中到15 mg / mL,并允许回收86%的注射蛋白质。

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