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首页> 外文期刊>Journal of Materials Chemistry, B. materials for biology and medicine >Self-recovering dual cross-linked hydrogels based on bioorthogonal click chemistry and ionic interactions
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Self-recovering dual cross-linked hydrogels based on bioorthogonal click chemistry and ionic interactions

机译：基于生物正交的双交联水凝胶自恢复双交联水凝胶，从而实现化学和离子相互作用

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摘要

The biocompatible, injectable and high water-swollen nature of hydrogels makes them a popular candidate to imitate the extracellular matrix (ECM) for tissue engineering bothin vitroandin vivo. However, commonly used covalently cross-linked hydrogels, despite their stability and tunability, are elastic and deteriorate as bulk material degrades which would impair proper cell function. To improve these deficiencies, here, we present a self-recovering cross-linked hydrogel formed instantaneously with functionalized poly(ethylene glycol) as a basis. We combine covalent cross-links introducedviaa strain-promoted azide-alkyne cycloaddition (SPAAC) click reaction and non-covalent links between phosphonate groups and calcium ions. By adjusting the ratios of non-covalent and covalent cross-links, we synthesized these dual cross-linked (DC) hydrogels that displayed storage moduli below similar to 2000 Pa and relaxation times from seconds to minutes. The gels recovered to 41-96% of their initial mechanical properties after two subsequent strain failures. Cryo-scanning electron microscopy revealed that DC hydrogels containing approximately equal amounts of covalent and non-covalent cross-links displayed phase separation. Finally, we functionalized the DC hydrogels by incorporating an integrin binding motif, RGDS, to provide a biocompatible environment for human mesenchymal stem cells (HMSCs) by facilitating adhesion inside the gel network. Inside these DC gels HSMCs displayed a viability up to 73% after five days of cell culture.
机译：水凝胶的生物相容性，可注射和高水溶性的性质使其成为模拟组织工程的细胞外基质（ECM）的受欢迎的候选者，这是玻体体内体内的组织工程。然而，常用的共价交联水凝胶，尽管它们的稳定性和可调性，是弹性的并且由于散装材料劣化而劣化，这会损害细胞功能。为了提高这些缺陷，在这里，我们提出了一种自回收交联水凝胶，其瞬间与官能化聚（乙二醇）形成为基础。我们结合共价十字链接引入viaa菌株促进的叠氮化物 - 炔环加油（Spaac）点击反应和膦酸盐基团之间的非共价连接。通过调节非共价和共价交联的比例，我们合成了这些双交联（DC）水凝胶，其低于2000Pa和弛豫时间从几秒到分钟的储存模量。在两次后续应变故障后，凝胶在其初始机械性能的41-96％中恢复至41-96％。冷冻扫描电子显微镜显示，DC水凝胶含有大约等量的共价和非共价交联显示相分离。最后，通过掺入整联蛋白结合基序，RGDS，通过促进凝胶网络内的粘附来提供对人间充质干细胞（HMSC）的生物相容性环境来官能化DC水凝胶。在这些DC凝胶中，HSMC在细胞培养的五天后显示出高达73％的活力。

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