...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Materials Chemistry, B. materials for biology and medicine >Non-invasive optical imaging of retinal A beta plaques using curcumin loaded polymeric micelles in APP(swe)/PS1(Delta E9)transgenic mice for the diagnosis of Alzheimer's disease
【24h】

Non-invasive optical imaging of retinal A beta plaques using curcumin loaded polymeric micelles in APP(swe)/PS1(Delta E9)transgenic mice for the diagnosis of Alzheimer's disease

机译：在APP（SWE）/ PS1（Delta E9）转基因小鼠中使用姜黄素负载聚合物胶束的视网膜β斑块的非侵入式光学成像用于诊断阿尔茨海默病的转基因小鼠

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Alzheimer's disease (AD) is a neurodegenerative disease clinically characterized by impaired memory and progressive cognitive decline. Despite the advances in AD research, an effective method to timely diagnose AD has remained elusive, and until now, most AD patients receive the available symptomatic treatments late. Although the pathological hallmarks of AD have been traditionally described in the brain, recent studies have shown similar pathological changes in the retina which is developmentally an extension of the forebrain. Interestingly, retinal beta-amyloid (A beta) accumulation preceded that of the brain in a transgenic mouse model of AD. In the quest of finding an early reliable biomarker for AD, researchers have targeted the optical imaging of retinal A beta plaques as a method of diagnosing AD. One promising polyphenol compound that has found application in this area is curcumin due to its natural binding affinity to A beta fibrils and oligomers while giving out a strong fluorescence signal. However, the clinical applications of curcumin have been difficult due to problems related to its bioavailability and retention in the body since it is a hydrophobic molecule. To address these limitations, we herein report the development of anionic and water-soluble DSPE-PEG(2000)curcumin polymeric micelles (also referred to as curcumin micelles) that can label both brain and retinal A beta plaquesex vivo.Following their intravitreal injection in the APP(swe)/PS1(Delta E9) transgenic mouse model of AD, green-labeled retinal deposits were optically imaged live using a rodent retinal microscope. Furthermore, these micelles had excellent intraocular biocompatibility, low hemolytic ratio, and were safe for use in two key retinal cell lines (ARPE-19 and 661W cells). Taken together, these findings provide an alternative insight into the optical imaging of A beta plaques for the diagnosis of AD using the eyes. More importantly, this study can be translated to humans in the future to improve on early diagnosis and timely management of the disease.
机译：阿尔茨海默病（AD）是一种神经变性疾病，其临床表征，记忆受损和逐渐认知下降。尽管广告研究进展，但要及时诊断广告的有效方法仍然难以捉摸，直到现在，大多数AD患者患有可用的症状治疗。虽然广告的病理标志传统上描述了大脑中，但最近的研究表明了视网膜中的类似病理变化，这是发育性的前脑的延伸。有趣的是，视网膜β-淀粉样蛋白（β）积累在AD的转基因小鼠模型中的脑中。在寻求AD的早期可靠的生物标志物中，研究人员靶向视网膜β斑块的光学成像作为诊断广告的方法。在该区域中发现施用的一种有前途的多酚化合物是由于其对β纤维和低聚物的天然结合亲和力而产生的姜黄素，同时发出强荧光信号。然而，由于与其生物利用度有关的问题和身体的保留，因此姜黄素的临床应用已经困难，因为它是疏水分子。为了解决这些限制，我们在本文中报告了阴离子和水溶性DSPE-PEG（2000）姜黄素聚合物胶束（也称为姜黄素胶束）的发育，其可以标记脑和视网膜β斑块βvivo。它们玻璃体内注射AD的AP（SWE）/ PS1（Delta E9）AD的转基因小鼠模型，使用啮齿动物视网膜显微镜进行光学成像。此外，这些胶束具有优异的眼内生物相容性，低溶血性比，并且在两个关键视网膜细胞系（ARPE-19和661W细胞）中是安全的。在一起，这些发现提供了一种替代地洞察β斑块的光学成像，用于使用眼睛诊断广告。更重要的是，该研究可以将来转化为人类，以提高早期诊断和及时管理疾病。

著录项

  • 来源
  • 作者

    Chibhabha Fidelis; Yang Yaqi; Ying Kuang; Jia Fujie; Zhang Qin; Ullah Shahid; Liang Zibin; Xie Muke; Li Feng;

  • 作者单位

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

    Sun Yat Sen Univ Zhongshan Sch Med Dept Anat &

    Neurobiol Guangzhou 510080 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号