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首页> 外文期刊>Biotechnology Progress >Application of Aurintricarboxylic Acid for the Adherence of Mouse P19 Neurons and Primary Hippocampal Neurons to Noncoated Surface in Serum-Free Culture
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Application of Aurintricarboxylic Acid for the Adherence of Mouse P19 Neurons and Primary Hippocampal Neurons to Noncoated Surface in Serum-Free Culture

机译：金三羧酸在无血清培养的未包被表面粘附小鼠P19神经元和海马原代神经元中的应用

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摘要

Dissociated primary neuron culture has been the most widely used model systems for neu-roscience research. Most of these primary neurons are cultured on adhesion matrix-coated surface to provide a proper environment for cell anchorage under serum-free conditions. In this study, we provide an alternative technique to promote the adhesions of these neurons using aurintricarboxylic acid (ATA), a nonpeptide compound, without surface manipulations. We first demonstrated that ATA could promote Chinese hamster ovary cell attachment and proliferation in serum-free medium in a dosage-dependent manner. We later showed that ATA significantly enhanced the attachment of the retinoic acid differentiated P19 mouse embryonal carcinoma (P19) neurons, with an optimal concentration around 30 μg/mL. A similar result was seen in primary hippocampal neurons, with an optimal ATA concentration around 15 μg/mL. Further morphological assessments revealed that the average neurite length and neuronal polarization were almost identical to that obtained using a conventional method with poly-L-lysine surface. The advantages of using the ATA treatment technique for immunochemical analysis are discussed.
机译：分离的原代神经元文化已成为神经科学研究中使用最广泛的模型系统。大多数这些原代神经元都在粘附基质涂层的表面上培养，以在无血清条件下为细胞锚定提供合适的环境。在这项研究中，我们提供了一种替代技术，可使用非肽化合物金三羧酸（ATA）来促进这些神经元的粘附，而无需进行表面处理。我们首先证明ATA可以以剂量依赖的方式促进无血清培养基中中国仓鼠卵巢细胞的附着和增殖。后来我们发现ATA可以显着增强视黄酸分化的P19小鼠胚胎癌（P19）神经元的附着，最佳浓度约为30μg/ mL。在原代海马神经元中观察到类似结果，最佳ATA浓度约为15μg/ mL。进一步的形态学评估表明，平均神经突长度和神经元极化几乎与使用聚L-赖氨酸表面的常规方法获得的平均神经突长度和神经元极化几乎相同。讨论了使用ATA处理技术进行免疫化学分析的优势。

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