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首页> 外文期刊>Biotechnology Progress >Bioenergetics and Pathway of Acid Blue 113 Degradation by Staphylococcus lentus
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Bioenergetics and Pathway of Acid Blue 113 Degradation by Staphylococcus lentus

机译：缓蚀葡萄球菌降解酸性蓝113的生物能及途径

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Bioreaction calorimetric studies of degradation of the dye acid blue 113 by Staphylococcus lentus are reported for the first time. The heat released during the dye degradation process can be successfully measured using reaction calorimeter. Power time and oxygen uptake rate (OUR) profile followed each other suggesting that heat profiles could monitor the progress of the dye degradation in biocalorimetry. The shifts observed in power-time profile indicated three distinct phases of the bioprocess indicating simultaneous utilization of glucose (primary) and dye (secondary carbon source). Secretion of azoreductase enzyme enhanced the degradation process. Optimization of aeration and agitation rates was observed to be vital to efficient dye degradation. The degradative pathway for acid blue 113 by S. lentus was delineated via high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FT-1R), and gas chromatography coupled with mass spectrometry (GC-MS) analyses. Interestingly the products of degradation were found to have low toxicity, as per cytotoxicity measurements.
机译：首次报道了由慢链球菌降解染料酸性蓝113的生物反应量热研究。染料降解过程中释放的热量可以使用反应量热仪成功测量。通电时间和氧气吸收率（OUR）曲线相互跟随，表明热量曲线可以监测生物量热法中染料降解的进程。功率时间曲线中观察到的变化表明生物过程的三个不同阶段，表明同时利用了葡萄糖（一级）和染料（二级碳源）。偶氮还原酶的分泌增强了降解过程。观察到通气和搅拌速率的优化对于有效的染料降解至关重要。通过高效液相色谱（HPLC），傅立叶变换红外光谱（FT-1R）和气相色谱-质谱联用（GC-MS）分析，确定了扁豆链霉菌对酸性蓝113的降解途径。有趣的是，根据细胞毒性测定，发现降解产物具有低毒性。

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