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首页> 外文期刊>Biotechnology Progress >Improvement of Mammalian Cell Culture Performance Through Surfactant Enabled Concentrated Feed Media
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Improvement of Mammalian Cell Culture Performance Through Surfactant Enabled Concentrated Feed Media

机译：通过启用表面活性剂的浓缩饲料培养基改善哺乳动物细胞培养性能

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摘要

The design of basal and feed media in mammalian cell culture is paramount towards ensuring acceptable upstream process performance in various operation modes, especially fed-batch culture. Mammalian cell culture media designs have evolved from the classical formulations designed by Eagle and Ham, to today's formulations designed from continuous improvement and statistical frameworks. Feed media is especially important for ensuring robust cell growth, productivity, and ensuring the product quality of recombinant therapeutics are within acceptable ranges. Numerous studies have highlighted the benefit of various media designs, supplements, and feed addition strategies towards the resulting cell culture process. In this work we highlight the use of a top-down level approach towards feed media design enabled by the use of select surfactants for the targeted enrichment of a chemically defined feed media. The use of the enriched media was able to improve product titers at g/L levels, without adversely impacting the growth of multiple Chinese Hamster Ovary cell lines or the product quality of multiple recombinant antibodies.
机译：哺乳动物细胞培养中基础培养基和饲料培养基的设计对于确保在各种操作模式下，尤其是分批补料培养中可接受的上游工艺性能至关重要。哺乳动物细胞培养基的设计已从Eagle和Ham所设计的经典配方演变为如今通过不断改进和统计框架而设计的配方。饲料培养基对于确保细胞强劲生长，提高生产率以及确保重组治疗剂的产品质量在可接受范围内尤其重要。大量研究强调了各种培养基设计，补充剂和饲料添加策略对最终细胞培养过程的好处。在这项工作中，我们重点介绍了采用自上而下的方法进行饲料介质设计，该方法通过使用精选的表面活性剂来实现化学成分确定的饲料介质的目标富集。使用富集培养基能够在g / L水平上提高产品滴度，而不会不利地影响多个中国仓鼠卵巢细胞系的生长或多个重组抗体的产品质量。

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