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Enzymatic Digestion of Alkaline-Sulflte Pretreated Sugar Cane Bagasse and Its Correlation with the Chemical and Structural Changes Occurring During the Pretreatment Step

机译:碱性硫酸盐预处理甘蔗渣的酶消化作用及其与预处理过程中化学和结构变化的关系

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摘要

Sugar cane bagasse is recalcitrant to enzymatic digestion, which hinders the efficient conversion of its polysaccharides into fermentable sugars. Alkaline-sulfite pretreatment was used to overcome the sugar cane bagasse recalcitrance. Chemical and structural changes that occurred during the pretreatment were correlated with the efficiency of the enzymatic digestion of the polysaccharides. The first 30 min of pretreatment, which removed approximately half of the initial lignin and 30% of hemicellulose seemed responsible for a significant enhancement of the cellulose conversion level, which reached 64%. After the first 30 min of pretreatment, delignification increased slightly, and hemicellulose removal was not enhanced; however, acid groups continued to be introduced into the residual lignin. Water retention values were 145% to the untreated bagasse and 210% to the bagasse pretreated for 120 min and fiber widths increased from 10.4 to 30 μm, respectively. These changes were responsible for an additional increase in the efficiency of enzymatic hydrolysis of the cellulose, which reached 92% with the 120 min pretreated sample.
机译:甘蔗渣对酶消化具有顽固性,这阻碍了其多糖向可发酵糖的有效转化。使用碱式亚硫酸盐预处理可克服甘蔗渣的顽固性。预处理期间发生的化学和结构变化与多糖的酶消化效率相关。预处理的前30分钟除去了大约一半的原始木质素和30%的半纤维素,这似乎是纤维素转化率达到64%的显着提高的原因。预处理的前30分钟后,去木质素略有增加,并且半纤维素的去除没有增强。然而,酸基继续被引入残留的木质素中。未经处理的甘蔗渣的保水值为145%,经120分钟预处理的甘蔗渣的保水值为210%,纤维宽度分别从10.4增加到30μm。这些变化导致纤维素酶水解效率的进一步提高,在120分钟的预处理样品中达到92%。

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