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Effect of Selected Aldehydes Found in the Corncob Hemicellulose Hydrolysate on the Growth and Xylitol Fermentation of Candida tropicalis

机译:玉米芯半纤维素水解物中某些醛对热带假丝酵母生长和木糖醇发酵的影响

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摘要

The effects of four aldehydes (furfural, 5-hydroxymethylfwfural, vanillin and syringalde-hyde), which were found in the corncob hemicellulose hydrolysate, on the growth and xylitol fermentation of Candida tropicalis were investigated. The results showed that vanillin was the most toxic aldehyde for the xylitol fermentation, followed by syringaldehyde, furfural and 5-hydroxymethylfurfural. Moreover, the binary combination tests revealed that furfural amplified the toxicity of other aldehydes and the toxicities of other binary combinations without furfural were simply additive. Based on the fermentation experiments, it was demonstrated that the inhibition of aldehydes could be alleviated by prolonging the fermentation incubation, increasing the initial cell concentration, enhancing the initial pH value and minimizing the furfural levels in the hydrolysate evaporation process. The strategies that we proposed to suppress the inhibitory effects of the aldehydes successfully avoided the complicated and costly detoxifications. Our findings could be potentially adopted for the industrial xylitol fermentation from hydrolysates.
机译:研究了玉米芯半纤维素水解产物中发现的四种醛(糠醛,5-羟甲基糠醛,香兰素和丁香醛)对热带假丝酵母生长和木糖醇发酵的影响。结果表明,香兰素是木糖醇发酵中毒性最高的醛,其次是丁二醛,糠醛和5-羟甲基糠醛。此外,二元组合测试表明糠醛会放大其他醛类的毒性,而其他没有糠醛的二元组合的毒性只是累加。基于发酵实验,证明了通过延长发酵温育,增加初始细胞浓度,提高初始pH值和最小化水解产物蒸发过程中的糠醛水平可以减轻醛的抑制。我们提出的抑制醛类抑制作用的策略成功地避免了复杂且昂贵的排毒。我们的发现可能被用于水解产物的工业木糖醇发酵。

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