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首页> 外文期刊>Biotechnology Progress >Novel Two-Stage Fermentation Process for Bioethanol Production Using Saccharomyces pastorianus
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Novel Two-Stage Fermentation Process for Bioethanol Production Using Saccharomyces pastorianus

机译：巴斯德酵母菌用于生物乙醇生产的新型两阶段发酵工艺

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Bioethanol produced from lignocellulosic materials has the potential to be economically feasible, if both glucose and xylose released from cellulose and hemicellulose can be efficiently converted to ethanol. Saccharomyces spp. can efficiently convert glucose to ethanol; however, xylose conversion to ethanol is a major hurdle due to lack of xylose-metabolizing pathways. In this study, a novel two-stage fermentation process was investigated to improve bioethanol productivity. In this process, xylose is converted into biomass via raon-Saccharomyces microorganism and coupled to a glucose-utilizing Saccharomyces fermentation. Escherichia coli was determined to efficiently convert xylose to biomass, which was then killed to produce E. coli extract. Since earlier studies with Saccharomyces pastorianus demonstrated that xylose isomer-ase increased ethanol productivities on pure sugars, the addition of both E. coli extract and xylose isomerase to S. pastorianus fermentations on pure sugars and corn stover hydrolysates were investigated. It was determined that the xylose isomerase addition increased ethanol productivities on pure sugars but was not as effective alone on the corn stover hydrolysates. It was observed that the E. coli extract addition increased ethanol productivities on both corn stover hydrolysates and pure sugars. The ethanol productivities observed on the corn stover hydrolysates with the E. coli extract addition was the same as observed on pure sugars with both E. coli extract and xylose isomerase additions. These results indicate that the two-stage fermentation process has the capability to be a competitive alternative to recombinant Saccharomyces cerevisiae-fowed fermentations.
机译：如果从纤维素和半纤维素释放的葡萄糖和木糖都可以有效地转化为乙醇，则由木质纤维素材料生产的生物乙醇具有经济上的可行性。酿酒酵母属。能有效地将葡萄糖转化为乙醇;然而，由于缺乏木糖代谢途径，木糖向乙醇的转化是主要障碍。在这项研究中，新的两阶段发酵过程进行了研究，以提高生物乙醇的生产率。在此过程中，木糖通过Raon-Saccharomyces微生物转化为生物质，并与利用葡萄糖的Saccharomyces发酵偶联。确定大肠杆菌可有效地将木糖转化为生物质，然后将其杀死以产生大肠杆菌提取物。由于早期对巴氏酵母的研究表明木糖异构酶提高了纯糖的乙醇生产率，因此对在纯糖和玉米秸秆水解产物上发酵巴氏酵母的大肠杆菌提取物和木糖异构酶都进行了研究。已确定木糖异构酶的添加增加了纯糖上乙醇的生产率，但单独对玉米秸秆水解产物没有那么有效。观察到，添加大肠杆菌提取物可提高玉米秸秆水解产物和纯糖的乙醇生产率。在添加大肠杆菌提取物的玉米秸秆水解产物中观察到的乙醇生产率与在添加大肠杆菌提取物和木糖异构酶的纯糖中观察到的乙醇生产率相同。这些结果表明，两阶段发酵过程具有竞争性地替代重组酿酒酵母发酵的能力。

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