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首页> 外文期刊>Biotechnology Progress >The Expression of the 14D9 Catalytic Antibody in Suspended Cells of Nicotiana tabacum Cultures Increased by the Addition of Protein Stabilizers and by Transference from Erlenmeyer Flasks to a 2-L Bioreactor
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The Expression of the 14D9 Catalytic Antibody in Suspended Cells of Nicotiana tabacum Cultures Increased by the Addition of Protein Stabilizers and by Transference from Erlenmeyer Flasks to a 2-L Bioreactor

机译：14D9催化抗体在烟草培养物悬浮细胞中的表达由于蛋白质稳定剂的添加和从锥形瓶向2-L生物反应器的转移而增加

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摘要

The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L~(-1) produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g LT gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% wlv inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
机译：分析了两种蛋白质稳定剂（聚乙烯吡咯烷酮[PVP]和明胶）对烟草细胞悬浮培养（Ab-KDEL和sec-Ab）生长和14D9产量的影响。在Ab-KDEL品系中，以1.0 g L〜（-1）的浓度添加PVP可产生最高的14D9总产量（生物质+培养基）（总可溶性蛋白[TSP]为4.82％）。添加明胶后，在5.0 g LT明胶中，Ab-KDEL品系获得了最高的14D9总产量（2.48％TSP）。当将Ab-KDEL悬浮细胞在2-L生物反应器中培养时，接种量为5％wlv时，最高14D9产量为8.1％TSP，这是迄今为止在测试平台上获得的最佳14D9产量（大肠杆菌，烟草叶和种子，烟草毛状根和细胞悬浮培养物。

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