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首页> 外文期刊>Journal of natural medicines >The origin identification method for crude drugs derived from arthropods and annelids using molecular biological techniques
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The origin identification method for crude drugs derived from arthropods and annelids using molecular biological techniques

机译：利用分子生物技术衍生自节肢动物和环菌的原产药物的原始鉴定方法

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We evaluated whether the origins of crude drugs derived from arthropods and annelids could be identified using molecular biological techniques. DNA was extracted from 20 crude drugs prepared from different animals using a commercial kit with added phenol treatment. The target regions used to identify origin were the mitochondrial 16S ribosomal RNA (rRNA), 12S rRNA, and cytochrome oxidase subunit I (COI) gene regions. Extracted DNA was amplified by polymerase chain reaction, and then sequenced by the Sanger method. The aligned sequences were compared with all available sequences using BLAST to estimate the origins of the crude drugs. The origin of crude drugs used in this study could be estimated using this method. The COI region was the best for identifying origin among three regions examined, based on the success rate of PCR amplification and analysis. Moreover, the 12S rRNA region was also useful for origin identification, with the exception of the earthworm. However, the origin of some crude drugs could not be strictly identified due to matches to various species in all three regions. One likely cause was that the species of origin of a crude drug has not been registered in DNA databases. We found that even the same crude drug from the same pharmaceutical company had different origins by production lot or import source country. Therefore, this method is useful not only for DNA-based origin identification but also quality control of production lots.
机译：我们评估了来自节肢动物和环状的粗药物的起源是否可以使用分子生物学技术来鉴定。使用具有添加苯酚处理的商业试剂盒从20只由不同动物制备的粗药物中提取的DNA。用于识别起源的目标区域是线粒体16S核糖体RNA（RRNA），12S rRNA和细胞色素氧化酶亚基I（COI）基因区域。通过聚合酶链式反应扩增提取的DNA，然后通过Sanger方法测序。将对齐的序列与使用爆炸的所有可用序列进行比较，以估计粗药物的起源。可以使用该方法估算本研究中使用的粗药物的起源。基于PCR扩增和分析的成功率，COI区域是最佳识别出现的原产地。此外，12S rRNA区域也可用于原产地鉴定，除蚯蚓外。然而，由于在所有三个地区的各种物种与各种物种的比赛，可能无法严格识别出一些粗药物的起源。一个可能的原因是粗药的原产地尚未在DNA数据库中登记。我们发现，即使来自同一制药公司的粗药物也是由生产批次或进口源国家的不同起源。因此，该方法不仅有用于基于DNA的原产地识别，而且是生产批次的质量控制。

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