...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Evaluation of an osmotic pump for microdialysis sampling in an awake and untethered rat.
【24h】

Evaluation of an osmotic pump for microdialysis sampling in an awake and untethered rat.

机译：唤醒和不受限化大鼠微透析抽样的渗透泵评价。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The feasibility of using an osmotic pump in place of a syringe pump for microdialysis sampling in rat brain was investigated. The use of an osmotic pump permits the rat to be free from the constraints of the standard tethered system. The in vitro flow rates of a microdialysis syringe pump (set at 10.80mul/h) and the osmotic pump (pump specifications were 11.35mul/h) with no probe attached were compared, yielding results of 10.87mul/h+/-1.7% and 10.95mul/h+/-8.0%, respectively. The average of four flow rate experiments in vivo yielded R.S.D.s less than 10% and an average flow rate of 11.1mul/h. Following the flow rate studies, in vivo sampling of neurotransmitters was accomplished with the osmotic pump coupled to a microdialysis probe implanted in the brain. Finally, after determination of basal levels of 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindole-3-acetic acid (5-HIAA) in the rats, the rats were dosed with benserazide followed by l-3,4-dihydroxyphenylalanine (l-DOPA). The results from the dosing study showed at least a 10-fold increase in compounds in the l-DOPA metabolic pathway (DOPAC and HVA) and a slight or no increase in 5-HIAA (serotonin metabolic pathway.) These results indicate that the osmotic pump is a viable alternative to the syringe pump for use in microdialysis sampling.
机译：研究了使用渗透泵的可行性代替用于大鼠大脑中微透析泵的注射器泵。使用渗透泵允许大鼠免于标准系绳系统的约束。微透析注射泵（设定为10.80mul / h）和渗透泵（泵规格为11.35mul / h）的体外流速，没有附着探针，产生10.87mul / h +/- 1.7％的结果10.95mul / h +/- 8.0％。体内四种流速实验的平均值产生少于10％，平均流量为11.1mul / h。在流动速率研究之后，在渗透到植入大脑中的微透析探针的渗透泵完成神经递质的体内采样。最后，在大鼠中测定3,4-二羟基苯基乙酸（DOPAC），同源甲酸（HVA）和5-羟基吲哚-3-乙酸（5-羟基吲哚-3-乙酸（5-羟基）的水平之后，用BENCERAZIDE提出大鼠。 L-3,4-二羟基苯丙氨酸（L-DOPA）。给药研究的结果表明，L-DOPA代谢途径（DOPAC和HVA）中的化合物的增量至少为10倍，并且在5-HIAA（血清素代谢途径中没有增加或没有增加。这些结果表明渗透泵是注射器泵的可行替代方案，用于微透析泵。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号