...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neuroscience Methods >Membrane configuration optimization for a murine in vitro blood-brain barrier model
【24h】

Membrane configuration optimization for a murine in vitro blood-brain barrier model

机译：鼠里鼠在体外血脑屏障模型中的膜配置优化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A powerful experimental tool used to study the dynamic functions of the blood-brain barrier (BBB) is an in vitro cellular based system utilizing cell culture inserts in multi-well plates. Currently, usage of divergent model configurations without explanation of selected variable set points renders data comparisons difficult and limits widespread understanding. This work presents for the first time in literature a comprehensive screening study to optimize membrane configuration, with aims to unveil influential membrane effects on the ability of cerebral endothelial cells to form a tight monolayer. First, primary murine brain endothelial cells and astrocytes were co-cultured in contact and non-contact orientations on membranes of pore diameter sizes ranging from 0.4. ??m to 8.0. ??m, and the non-contact orientation and smallest pore diameter size were shown to support a significantly tighter monolayer formation. Then, membranes made from polyethylene terephthalate (PET) and polycarbonate (PC) purchased from three different commercial sources were compared, and PET membranes purchased from two manufacturers facilitated a significantly tighter monolayer formation. Models were characterized by transendothelial electrical resistance (TEER), sodium fluorescein permeability, and immunocytochemical labeling of tight junction proteins. Finally, a murine brain endothelial cell line, bEnd.3, was grown on the different membranes, and similar results were obtained with respect to optimal membrane configuration selection. The results and methodology presented here on high throughput 24-well plate inserts can be translated to other BBB systems to advance model understanding. ? 2012 Elsevier B.V.
机译：用于研究血脑屏障（BBB）的动态功能的强大实验工具是利用多孔板中的细胞培养件的体外细胞基体系。目前，在没有说明所选变量设定点的情况下使用不同模型配置呈现数据比较困难并限制广泛的理解。这项工作主要是文献中的第一次综合筛选研究，优化膜配置，旨在揭示有影响力的膜对脑内皮细胞形成紧密单层的能力。首先，在孔径尺寸范围为0.4的孔径尺寸的膜上的接触和非接触方向上共培养初级鼠脑内皮细胞和星形胶质细胞。 ?? m到8.0。 [? m，并且显示不接触方向和最小的孔径尺寸，以支持显着更严格的单层形成。然后，比较了由三种不同商业来源购买的聚对苯二甲酸乙二醇酯（PET）和聚碳酸酯（PC）制成的膜，并从两家制造商购买的PET膜促进了显着更严格的单层形成。模型的特征在于经过术语电阻（Teer），荧光素渗透性和紧密结蛋白的免疫细胞化学标记。最后，在不同的膜上生长鼠脑内皮细胞系弯曲，并且相对于最佳膜构型选择获得类似的结果。这里呈现在高吞吐量24孔板插入件上的结果和方法可以转换为其他BBB系统以推进模型理解。还2012年Elsevier B.v.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号