...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of optics >Multispectral photoacoustic imaging of tumours in mice injected with an enzyme-activatable photoacoustic probe
【24h】

Multispectral photoacoustic imaging of tumours in mice injected with an enzyme-activatable photoacoustic probe

机译：用酶活化光声探针注射小鼠肿瘤的多光谱光声成像

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Photoacoustic (PA) imaging offers depth-resolved images of optical absorbers with the spatial resolution of ultrasound imaging. To enhance tumour contrast, tumour-specific probes are used as contrast agents. We synthesised a colourless PA probe that is activated in the presence of gamma-glutamyltranspeptidase, a cancer-associated enzyme, to show its original colour and fluorescence. We have acquired high specificity fluorescence images of small tumours, using a fluorescent probe based on similar enzymatic reactions. Here, we developed a PA imaging technique to detect the PA probe. In PA imaging, depending on the concentration and excitation wavelength of the probe, the intensities of the probe signals may be lower than those of the background signals produced by intrinsic optical absorbers such as haemoglobin. For probe imaging in the presence of strong background signals, multispectral photoacoustic (MS-PA) imaging was evaluated. In MS-PA imaging, the spectral fitting method, which distinguishes the probe signals from background signals using reference spectra, has been widely used. To compensate for the decrease of fluence due to optical attenuation in biological tissue, we used a simplified compensation method that calculates fluence inside biological tissues by the Monte-Carlo model using published data on optical properties of biological tissues. The validity of the method was confirmed using tissue-mimicking phantoms. Finally, MS-PA imaging of a mouse subcutaneous tumour injected with the activatable probe was demonstrated. In conclusion, our MS-PA imaging technique afforded successful detection of the activated probe in the tumour, and time-increase of PA signals were successfully observed.
机译：光声（PA）成像提供具有超声成像的空间分辨率的光学吸收器的深度分辨图像。为了增强肿瘤对比，肿瘤特异性探针用作造影剂。我们合成无色PA探针，在γ-谷氨酰胺转移酶，癌症相关酶的存在下被激活，以显示其原始颜色和荧光。我们使用基于类似酶促反应的荧光探针获得了小肿瘤的高特异性荧光图像。在这里，我们开发了一种PA成像技术来检测PA探头。在PA成像中，根据探针的浓度和激发波长，探针信号的强度可以低于由内在光学吸收剂（例如血红蛋白）产生的背景信号的强度。对于存在强大的背景信号存在的探针成像，评估多光谱光声（MS-PA）成像。在MS-PA成像中，广泛使用了使用参考光谱与背景信号区别于使用参考光谱的光谱拟合方法。为了补偿由于生物组织中的光学衰减引起的注量，我们使用了一种简化的补偿方法，通过Monte-Carlo模型使用发表的生物组织的光学性质来计算生物组织内的流量。使用组织模拟幻像确认该方法的有效性。最后，证明了用可活化探针注射的小鼠皮下肿瘤的MS-PA成像。总之，我们的MS-PA成像技术得到了成功地检测肿瘤中活化的探针，并且成功地观察到PA信号的时间增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号