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首页> 外文期刊>Journal of Molecular Neuroscience: MN >Phenotypic Differences in Primary Murine Microglia Treated with NOD1, NOD2, and NOD1/2 Agonists
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Phenotypic Differences in Primary Murine Microglia Treated with NOD1, NOD2, and NOD1/2 Agonists

机译：用NOD1，NOD2和NOD1 / 2激动剂治疗原发性鼠髓的表型差异

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The purpose of the study was studying the influence of different NOD agonists on the morphological phenotype of primary murine microglia and to examine their influence on characteristic cytokines. Primary CD11b-positive cells were isolated from the brain of neonatal mice. The microglial phenotype of the cells was examined by ionized calcium-binding adapter molecule (Iba)1 staining. After14 days in culture, these cells were stimulated by iE-DAP, L18-MDP, or M-TriDAP as NOD1, NOD2, and NOD1/2 agonists, respectively. The cellular morphology was recorded and compared to the phenotype of cells cultured in medium alone or after LPS stimulation. The cells developed a specific phenotype only after treatment with the NOD2 agonist L18-MDP. These cells were characterized by straight extensions carrying tiny spikes and had a high ramification index. This was in sharp contrast to all other treatments, which always resulted in an amoeboid phenotype typically shown by activated microglia in vivo and by cultured microglia in vitro. The staining intensity of IL-6 and TNF-alpha did not reveal any clear difference independent of the NOD agonist treatment. In contrast, an increased staining intensity was observed for IL-10 after L18-MDP treatment. The NOD2 agonist L18-MDP induced a morphologically distinct phenotype characterized by microspike-decorated dendritiform extensions and a high degree of ramification in primary murine microglia. Increased ramification index and elevated staining intensity of anti-inflammatory IL-10 as hallmarks suggest that a M2-like phenotype of microglia was induced.
机译：该研究的目的是研究不同点头激动剂对原发性小鼠小胶质细胞的形态表型的影响，并检查它们对特征细胞因子的影响。原发性CD11b阳性细胞与新生儿小鼠的脑中分离出来。通过电离钙结合衔接子分子（IBA）1染色来检查细胞的小胶质表型。在培养14天后，分别通过IE-DAP，L18-MDP或M-Tridap刺激这些细胞作为NOD1，NOD2和NOD1 / 2激动剂。记录细胞形态，并与单独或在LPS刺激之后在培养基中培养的细胞表型进行比较。仅在用Nod2激动剂L18-MDP处理后，该细胞仅在处理后开发特定表型。这些细胞的特征在于携带微小尖峰的直延伸并具有高的分枝指数。与所有其他治疗鲜明对比，这总是导致通常通过体内活化的微胶质细胞和体外培养的微胶质显示的作物泛表型。 IL-6和TNF-α的染色强度没有明显无关的任何明显的差异，与点头激动剂治疗无关。相反，在L18-MDP处理之后，观察到IL-10的增加的染色强度。 Nod2激动剂L18-MDP诱导形态学上明显的表型，其特征在于Microprike装饰的树突式延伸和初级鼠小胶质细胞的高度分枝。随着标志性的，抗炎IL-10的增加的分枝指数和升高的染色强度表明，诱导了微胶质细胞的M2样表型。

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