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首页> 外文期刊>Journal of orthopaedic research >Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration
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Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration

机译：针刺损伤导致椎间盘退变小鼠模型中的急性和长期的机械缺乏

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摘要

Low back pain is a significant socioeconomic burden and intervertebral disc degeneration has been implicated as a cause. A reliable animal model of disc degeneration is necessary to evaluate therapeutics, and functional metrics are essential to quantify their benefit. To this end, needle puncture injuries were created in the caudal intervertebral discs of mice to induce disc degeneration. Compression, torsion, and creep mechanics were assessed both immediately and after eight weeks to distinguish between the effects of injury and the subsequent reparative or degenerative response. Two needle sizes (29 and 26 gauge) were used to determine injury size-dependence. Compressive stiffness (62%), torsional stiffness (60%), and early damping stiffness (84%) decreased immediately after injury with the large needle (26G). These mechanical properties did not change over time despite structural and compositional changes. At 8 weeks following large needle injury, disc height decreased (37%), nucleus pulposus (NP) glycosaminoglycan content decreased (41%), and NP collagen content increased (45%). The small needle size had no significant effect on mechanics and did not initiate degenerative changes in structure and composition. Thus, the injection of therapeutics into the NP with a minimal needle size may limit damage due to the needle insertion. These findings, along with the wide commercial availability of mouse-specific biological probes, indicate that the mouse caudal disc model can be a powerful tool for investigating disc degeneration and therapy.
机译：低腰疼痛是一个重要的社会经济负担，椎间盘退变已被牵连作为原因。可靠的椎间盘退变动物模型是评估治疗方法所必需的，并且功能度量对于量化其利益至关重要。为此，在小鼠的尾部椎间盘中产生针刺损伤以诱导椎间盘变性。在八周后立即进行压缩，扭转和蠕变力学，以区分损伤的影响和随后的重复或退行性反应。两个针尺寸（29和26尺）用于确定伤害尺寸依赖性。压缩刚度（62％），扭转刚度（60％）和早期阻尼刚度（84％）与大针（26g）损伤后立即降低。尽管结构性和组成变化，这些机械性能随着时间的推移而没有改变。在大针损伤后8周，圆盘高度减少（37％），髓髓（NP）糖胺聚糖含量降低（41％），NP胶原蛋白含量增加（45％）。小针尺寸对力学没有显着影响，并且没有启动结构和组成的退行性变化。因此，用最小针尺寸将治疗剂注入NP可以限制由于针插入而损坏。这些发现以及小鼠特异性生物探针的广泛商业可用性，表明小鼠尾部光盘模型可以是调查光盘变性和治疗的强大工具。

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