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首页> 外文期刊>Journal of orthopaedic research >Effect of chondroitinase ABC on adhesion and behavior of synovial membrane-derived mesenchymal stem cells in rabbit partial-thickness chondral defects
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Effect of chondroitinase ABC on adhesion and behavior of synovial membrane-derived mesenchymal stem cells in rabbit partial-thickness chondral defects

机译：软骨素酶ABC对滑膜膜衍生间充质干细胞粘附及行为的影响兔偏厚孔缺损

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Transplanted cells may have difficulty attaching to the surface of partial-thickness chondral lesions because of the anti-adhesive properties of the proteoglycan rich matrix. Therefore, the current study attempts to evaluate the effect of chondroitinase ABC (chABC) on the adhesion and behavior of transplanted synovial membrane-derived mesenchymal stem cells (SDSCs) in rabbit partial-thickness chondral defects. In ex vivo adhesion experiments, chABC treatment (0.1 U/ml) was increased in SDSC attachment to the cartilage explants, and significantly diminished by pretreatment with neutralizing antibody against fibronectin. In the in vivo experiments, 1 day and 4 weeks after the chABC treatment (0.1 and 1 U/ml), the immunoreactivity (IR) against CS-56 (intact chondroitin sulfate antibody) was markedly decreased; however, the IR of 2B6 (stub of the chondroitin 4-sulfate chain), 3B3 (stub of the chondroitin 6-sulfate chain), and fibronectin was increased. At 12 weeks, this IR returned to normal except in the high-dose chABC-treated group (1 U/ml). Furthermore, the attachment of SDSCs to the chondral defects after chABC treatment was increased at 7 days compared with that in the chondral defects pretreated with saline. However, the tissue repaired by SDSCs was negatively stained for type II collagen at 12 weeks. In conclusion, these results showed that the exposure to fibronectin by chABC treatment enhances the attachment of SDSCs to partial-thickness chondral defects. However, the tissue regenerated by SDSCs showed lack of hyaline cartilage regeneration. Thus, to understand the fate of transplanted MSCs in cartilage defect is very important for successful cell therapies.
机译：由于蛋白多糖的富集基质的抗粘接性能，移植的细胞可能难以附着到部分厚度骨性病变的表面。因此，目前的研究试图评估软骨素酶ABC（CHABC）对兔部分厚度缺陷中移植的滑膜膜衍生间充质干细胞（SDSC）的粘附性和行为的作用。在离体粘附实验中，CHABC处理（0.1U / mL）在软骨外植物的SDSC附着中增加，并通过对纤连蛋白的中和抗体预处理显着减少。在体内实验中，CHABC处理（0.1和1 U / mL）后1天和4周，对CS-56的免疫反应性（IR）显着降低;然而，增加了2b6（软骨素4-硫酸根链条链的短截线），3b3（软骨素6-硫酸盐链的短截线）和纤连蛋白。在12周时，除了高剂量CHABC处理组（1U / mL）中，该IR返回正常。此外，与用盐水预处理的骨质缺陷相比，在7天内增加了SDSC在CHABC处理后的骨质缺陷。然而，由SDSCS修复的组织在12周时为II型胶原染色。总之，这些结果表明，通过CHABC处理暴露于纤维素素，增强了SDSCs对部分厚度骨质缺陷的附着。然而，SDSC再生的组织显示出缺乏透明软骨再生。因此，为了理解软骨缺陷中移植的MSCs的命运对于成功的细胞疗法非常重要。

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