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首页> 外文期刊>Journal of orthopaedic research >Reduction in Pseudomonas aeruginosa and Staphylococcus aureus biofilms from implant materials in a diffusion dominated environment
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Reduction in Pseudomonas aeruginosa and Staphylococcus aureus biofilms from implant materials in a diffusion dominated environment

机译：从扩散占主导地位的环境中从植入物材料中减少假单胞菌铜绿假单胞菌和金黄色葡萄球菌生物膜

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Antibiotic-loaded calcium sulfate beads (CS-B) are used to treat biofilm-related periprosthetic joint infections (PJI). A previous study has shown that such beads are effective in reducing lawns biofilms grown on agar plates; however, the ability of CS-B to eradicate biofilms grown on solid orthopedic material surfaces has not been investigated. We grew biofilms of bioluminescent strains of Pseudomonas aeruginosa Xen41 and a USA300 MRSA Staphylococcus aureus SAP231 on ultra-high molecular weight polyethylene (PE), hydroxyapatite (HA), and 316L stainless steel (SS) coupons for three days under static growth conditions, with daily nutrient exchange. The coupons were rinsed with sterile phosphate buffered saline (PBS) to remove planktonic bacteria and placed in a petri dish, surrounded by four either antibiotic vancomycin and tobramycin loaded (CS-BV+T) or unloaded beads (CS-B-U). A thin layer of agar was overlaid to simulate a periprosthetic infection where an implant abuts soft tissue and then incubated for 72 h. The amount of biofilm was measured by bioluminescence imaging (BLI) for activity and viable cell count (CFUs). Coupons exposed to CS-BV+T showed a significant reduction in the amount of biofilm within 24 h, regardless of the bacterial strain or material type. The coupons exposed to control CS-B-U had no effect on bacteria over 72 h. Statement of Clinical Significance: Antibiotic-loaded calcium sulfate beads (CS-B) were effective in significantly reducing mature biofilms of P. aeruginosa and S. aureus from orthopedic relevant surfaces in our in vitro agar model. (c) 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:3081-3085, 2018.
机译：抗生素负载硫酸钙珠粒（CS-B）用于治疗生物膜相关的Periprosithetth感染（PJI）。先前的研究表明，这种珠子在减少在琼脂平板上生长的草坪生物膜有效;然而，尚未研究CS-B将CS-B根除生物膜的能力尚未得到研究生长在固体矫形材料表面上生长的能力。在静态生长条件下，我们在超高分子量聚乙烯（PE），羟基磷灰石（PE），羟基磷灰石（HA）和316L不锈钢（HA）和316L不锈钢（SS）试样上延长了铜绿假单胞菌Xen41和USA300 MRSA金黄色葡萄球菌SAP231的生物膜。每日营养交换。优惠券用无菌磷酸盐缓冲盐水（PBS）冲洗，以除去浮游细菌并置于培养皿中，围绕四种抗生素万古霉素和含有烟霉素（Cs-BV + T）或未加载的珠粒（Cs-B-U）。重叠薄的琼脂层以模拟植入物邻接软组织然后孵育72小时的植物瘤细胞感染。通过生物发光成像（BLI）测量生物膜的量，用于活性和活细胞计数（CFU）。无论细菌菌株或材料类型如何，暴露于CS-BV + T的优惠券显示出24小时内的生物膜的量显着降低。暴露于控制CS-B-U的优惠券对细菌的影响超过72小时。临床意义陈述：抗生素加载的硫酸钙珠粒（CS-B）可有效减少来自我们在体外琼脂模型中的骨科相关表面的P.铜绿假单胞菌和金黄色葡萄球菌的成熟生物膜。 （c）2018年骨科研究会。由Wiley Hearygers，Inc.J orthop Res 36：3081-3085,2018出版。

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