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首页> 外文期刊>Journal of Photochemistry and Photobiology, B. Biology: Official Journal of the European Society for Photobiology >Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition
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Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition

机译：Falcaria寻常叶片提取缀合的铜纳米粒子的新型合成具有有效的细胞毒性，抗氧化剂，抗真菌，抗菌和皮肤伤口和皮肤伤口愈合活性在体外和体内病症

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Facile green synthesis of copper nanoparticles from different biological procedures has been indicated, but among all, biosynthesis of copper nanoparticles from medicinal plants is considered as the most suitable method. The use of medicinal plant material increases the therapeutical effects of copper nanoparticles. The aim of this study was green synthesis of copper nanoparticles from aqueous extract of Falcaria vulgaris leaf (CuNPs) and assessment of their cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing properties. These nanoparticles were characterized by X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) analysis. The synthesized CuNPs had great cell viability dose-dependently (Investigating the effect of the CuNPs on human umbilical vein endothelial cell (HUVEC) line) and indicated this method was nontoxic. Also, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was done to assess the antioxidant activities, which indicated similar antioxidant potentials for CuNPs and butylated hydroxytoluene. In part of cutaneous wound healing property of CuNPs, after creating the cutaneous wound, the rats were randomly divided into six groups: treatment with 0.2% CuNPs ointment, treatment with 0.2% CuSO4 ointment, treatment with 0.2% F. vulgaris ointment, treatment with 3% tetracycline ointment, treatment with Eucerin basal ointment, and untreated control. These groups were treated for 10 days. Treatment with CuNPs ointment remarkably increased (p <= .01) the wound contracture, vessel, hexosamine, hydroxyl proline, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and substantially reduced (p <= .01) the wound area, total cells, neutrophil, and lymphocyte compared to other groups. In antibacterial and antifungal parts of this research, the concentration of CuNPs with minimum dilution and no turbidity was considered minimum inhibitory concentration (MIC). To determine minimum fungicidal concentration (MFC) and minimum bactericidal concentration (MBC), 60 mu L MIC and three preceding chambers were cultured on Sabouraud Dextrose Agar and Muller Hinton Agar, respectively. The minimum concentration with no fungal and bacterial growth were considered MFC and MBC, respectively. CuNPs inhibited the growth of all fungi at 2-4 mg/mL concentrations and removed them at 4-8 mg/mL concentrations (p <= .01). In case of antibacterial effects of CuNPs, they inhibited the growth of all bacteria at 2-8 mg/mL concentrations and removed them at 4-16 mg/mL concentrations (p <= .01). The results of XRD, FT-IR, UV, TEM, and FE-SEM confirm that the aqueous extract of F. vulgaris leaf can be used to yield copper nanoparticles with notable amount of antioxidant, antifungal, antibacterial, and cutaneous wound healing potentials without any cytotoxicity. Further clinical trials are necessary for confirmation these therapeutical effects of CuNPs in human.
机译：已经表明了铜纳米粒子的铜纳米粒子的体积形成，但其中，来自药用植物的铜纳米粒子的生物合成被认为是最合适的方法。使用药用植物材料增加了铜纳米粒子的治疗作用。本研究的目的是来自紫色叶片（CUNP）水提取物的铜纳米粒子的绿色合成，并评估其细胞毒性，抗氧化剂，抗真菌，抗菌和皮肤伤口愈合性能。这些纳米颗粒的特征在于X射线衍射（XRD），傅里叶变换红外光谱（FT-IR），紫外 - 可见光谱（UV），透射电子显微镜（TEM）和场发射扫描电子显微镜（Fe-SEM）分析。合成的CUNP具有很大的细胞活力剂剂量依赖性（研究CUNP对人脐静脉内皮细胞（HUVEC）线的影响），并表明该方法是无毒的。此外，进行2,2-二苯基-1-富铬酰基（DPPH）试验以评估抗氧化活性，表明CUNP和丁基化羟基甲苯的相似抗氧化势。其中，在CUNP的皮肤伤口愈合特性中，在创造皮肤伤口后，将大鼠随机分为6组：用0.2％CUNPS软膏治疗，用0.2％CUSO4软膏治疗，用0.2％F.寻常软膏处理，治疗3％四环素软膏，用EUCERIN基础软膏治疗，并进行未处理的对照。这些群体治疗10天。用CUNPS软膏治疗显着增加（p <= .01）伤口挛缩，容器，六甲胺，羟脯氨酸，六尿酸，纤维纤维和纤维细胞/成纤维细胞/成纤维细胞，并且基本上还原（p <= .01）伤口区域，总细胞，与其他组相比，中性粒细胞和淋巴细胞。在本研究的抗菌和抗真菌部分中，CUNP的浓度具有最小稀释，并且没有浊度被认为是最小抑制浓度（MIC）。为了确定最小的杀菌浓度（MFC）和最小杀菌浓度（MBC），分别在Sabouraucud葡萄糖琼脂和Muller Hinton琼脂上培养60μlMIC和三个前室。没有真菌和细菌生长的最小浓度分别被认为是MFC和MBC。 CUNP抑制了2-4mg / ml浓度下所有真菌的生长，并以4-8mg / ml浓度除去它们（P <= .01）。在CUNP的抗菌作用的情况下，它们抑制了2-8mg / ml浓度下所有细菌的生长，并以4-16mg / ml浓度除去它们（P <= .01）。 XRD，FT-IR，UV，TEM和Fe-SEM的结果证实了F.Vulgaris叶的水提取物可用于产生具有显着抗氧化剂，抗真菌，抗菌和皮肤伤口愈合潜力的铜纳米粒子而没有任何细胞毒性。进一步的临床试验对于确认CUNPS在人体中的这些治疗作用是必要的。

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