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首页> 外文期刊>Journal of Photochemistry and Photobiology, B. Biology: Official Journal of the European Society for Photobiology >Silver nanoparticles synthesis from Bacillus sp KFU36 and its anticancer effect in breast cancer MCF-7 cells via induction of apoptotic mechanism
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Silver nanoparticles synthesis from Bacillus sp KFU36 and its anticancer effect in breast cancer MCF-7 cells via induction of apoptotic mechanism

机译：银纳米粒子通过诱导凋亡机制的乳腺癌MCF-7细胞中的抗癌效应及其抗癌作用及其抗癌作用

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摘要

Biological synthesis of nanoparticles is a growing research trend because it has numerous applications in pharmaceutics and biomedicine. The aim of this study was to obtain silver nanoparticles (AgNPs) from Bacillus sp. KFU36, a marine strain, and to assess its anticancer activity. The supernatant of Bacillus sp. KFU36 was supplemented with silver nitrate and the nanoparticles obtained were characterized spectrophotometrically and microscopically. A band of surface plasmon resonance was appeared at 430 nm, as revealed by UV-vis spectrophotometry. X-ray diffraction spectrum and Energy Dispersive Spectroscopy confirmed the crystalline and metallic structure of the AgNPs, respectively. Scanning electron microscopy revealed that the shape of the synthesized AgNPs were spherical and the size extended between 5 and 15 nm. The AgNPs were investigated for their potential anticancer effects on the cell viability, migration and apoptosis using MTT and wound-healing assays, and flow cytometry, respectively. The cytotoxic effects of these nanoparticles were evidenced by the decreasing the cell viability (as 15% at 50 mu g/ml), cell density, adhesion capacity and losing the normal shape and size, and inducing the apoptosis on MCF-7 by 61% at 50 mu g/ml. These findings confirm that the synthesized AgNPs exhibited superior anticancer activities and therefore could be exploited as a promising, cost-effective, and environmentally benign strategy in treating this disease in future.
机译：纳米粒子的生物合成是一种日益增长的研究趋势，因为它在药物和生物医学中具有许多应用。本研究的目的是从芽孢杆菌获得银纳米颗粒（AgNP）。 KFU36，海洋菌株，并评估其抗癌活动。芽孢杆菌的上清液。 KFU36补充有硝酸银，得到的纳米颗粒分光光度和显微镜。在430nm处出现一条表面等离子体共振，如UV-Vis分光光度法所揭示的。 X射线衍射光谱和能量色散光谱分别证实了AgNP的结晶和金属结构。扫描电子显微镜显示，合成的AgNP的形状是球形的，尺寸在5到15nm之间延伸。使用MTT和伤口愈合测定和流式细胞术分别研究了对细胞活力，迁移和细胞凋亡的潜在抗癌的影响。通过降低细胞活力（为50μmg/ ml），细胞密度，粘附容量和失去正常形状和尺寸，并诱导MCF-7的细胞凋亡，通过减少细胞活力（为50μg/ ml），对MCF-7的细胞凋亡进行了证明了这些纳米颗粒的细胞毒性作用在50 mu g / ml。这些调查结果证实，合成的AGNPS表现出优越的抗癌活动，因此可以被利用作为未来治疗这种疾病的有前途，经济效益和环境良性的策略。

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