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首页> 外文期刊>Journal of proteomics >Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration
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Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration

机译：鹿鹿茸干细胞定量蛋白质组学分析作为哺乳动物器官再生模型

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摘要

The ability to activate and regulate stem cells during wound healing and tissue/organ regeneration is a promising field which could bring innovative approaches to regenerative medicine. The regenerative capacity of invertebrates has been well documented, however in mammals, stem cells that drive organ regeneration are rare. Deer antler is unique in providing a mammalian model of complete organ regeneration based on stem cells. The present study investigated the differentially regulated proteins (DRPs) between different antler stem cell populations (n = 3) using 2D-DIGE. Western blotting was used to validate the proteomics results. Comparative proteomics resulted in protein profiles which were similar for the biological replicates but different between the cells derived from two different stem cell niches involved in antler growth/regeneration and cells derived from facial periosteum. Ninety-two up- and down-regulated proteins were identified by MALDI-TOF MS. The work indicates that the epithelial-mesenchymal transition process may participate in the initiation of antler regeneration including the first stage of scar-less wound healing. Cell mobility is also highly regulated during antler regeneration. Energy and nucleotide metabolism may however be less active in antler regeneration as compared to that in antler generation phase. These results provide new insights into the underlying mechanisms of stem cell-based regeneration of mammalian organs.
机译：在伤口愈合和组织/器官再生期间激活和调节干细胞的能力是一个有前景的领域，可以带来创新的再生医学方法。无脊椎动物的再生能力已经充分记录，但在哺乳动物中，驱动器官再生的干细胞是罕见的。鹿鹿茸在提供基于干细胞的完全器官再生哺乳动物模型方面是独一无二的。本研究研究了使用2D-Dige的不同鹿茸干细胞群（n = 3）之间的差异调节的蛋白质（DRP）。 Western Blotting用于验证蛋白质组学结果。对比蛋白质组学导致蛋白质谱类似于生物复制，但是衍生自参与鹿茸生长/再生的两种不同干细胞核桃的细胞与来自面部肝脏的细胞之间的细胞之间的不同。 MALDI-TOF MS鉴定了92个上调和下调的蛋白质。该作品表明上皮 - 间充质过渡过程可以参与鹿茸再生的启动，包括疤痕伤口愈合的第一阶段。在鹿角再生期间，细胞迁移率也高度调节。然而，与鹿角生成阶段相比，能量和核苷酸代谢在鹿茸再生中可能不那么活跃。这些结果对哺乳动物器官的干细胞再生的潜在机制提供了新的见解。

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