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首页> 外文期刊>Journal of plant nutrition and soil science >Spatially tracking carbon through the root-rhizosphere-soil system using laser ablation-IRMS
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Spatially tracking carbon through the root-rhizosphere-soil system using laser ablation-IRMS

机译：使用激光烧蚀 - IRMS通过根根际 - 土壤系统进行空间跟踪碳

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摘要

The intimate relationships between plant roots, rhizosphere, and soil are fostered by the release of organic compounds from the plant into soil through various forms of rhizodeposition and the simultaneous harvesting of nutrients from the soil to the plant. Here we present a method to spatially track and map the migration of plant-derived carbon (C) through roots into the rhizosphere and surrounding soil using laser ablation-isotope ratio mass spectrometry (LA-IRMS). We used switchgrass microcosms containing soil from field plots at the Kellogg Biological Station (Hickory Corners, Michigan, USA) which have been cropped with switchgrass since 2008. We used a (CO2)-C-13 tracer to isotopically label switchgrass plants for two diel cycles and tracked subsequent movement of labeled C using the spatially specific (< 100 mu m resolution) delta C-13 analysis enabled by LA-IRMS. This approach permitted assessment of variable C flow through different roots and enabled mapping of spatial variability of C allocation to the rhizosphere. Highly C-13-enriched C (consistent with production during the (CO2)-C-13 application period) extended approximate to 0.5-1 mm from the root into the soil, suggesting that the majority of recent plant-derived C was within this distance of the root after 48 h. Tracking the physical extent of root exudation into the rhizosphere can help evaluate the localization of plant-microbe interactions in highly variable subsurface environments, and the use of the isotopic label can differentiate freshly fixed C (presumably from root exudates) from other types of subsurface C (e.g., plant necromass and microbial turnover). The LA-IRMS technique may also serve as a valuable screening technique to identify areas of high activity for additional microbial or geochemical assays.
机译：通过各种形式的无状沉积，通过各种形式的无状沉积和从土壤中的营养成分释放到土壤中的有机化合物与土壤中的有机化合物释放到植物中的有机化合物之间的亲密关系。在这里，我们使用激光烧蚀同位素比质谱（La-IRMS）来介绍空间轨道和植物衍生的碳（C）通过根部迁移到根际和周围土壤中的方法。我们使用了从2008年以来的kellogg生物站（山核桃角落，密歇根州，Michigan，USA）的场地块的SwitchGrass微观含有土壤的田间地块。我们使用了（二氧化碳）-C-13示踪剂对两国DIEL的同位素标记开关植物使用LA-IRMS启用的空间特异性（<100 mu m分辨率）ΔC-13分析，循环和跟踪的标记C的随后移动。这种方法允许通过不同根部的变量C流量进行评估，并使C分配的空间可变性的映射到根际。富含C-13富集的C（在（二氧化碳）-C-13施用期间的生产一致）将距离根部延伸至0.5-1毫米到土壤中，表明最近的大多数植物衍生的C都在这内48小时后根的距离。跟踪根除根渗入根际的物理范围可以帮助评估高度可变地下环境中的植物微生物相互作用的定位，并且同位素标签的使用可以从其他类型的地下C分化新鲜固定的C（可能是从根出渗出物） （例如，植物坏组织和微生物周转）。 LA-IRMS技术还可以用作有价值的筛选技术，以识别用于额外的微生物或地球化学测定的高活性区域。

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