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首页> 外文期刊>American Journal of Dermatopathology >Immunohistochemistry in Dermatopathology: A Retrospective Study of the Most Frequently Used Antibodies
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Immunohistochemistry in Dermatopathology: A Retrospective Study of the Most Frequently Used Antibodies

机译：皮肤病理学中的免疫组织化学：最常用抗体的回顾性研究

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摘要

Immunohistochemistry (IHC) is an ancillary technique to improve diagnostic accuracy and prognosis in histopathology of both inflammatory and neoplastic cutaneous disorders. However, only a few studies address specifically the set of antibodies available for inflammatory or neoplastic skin diseases. In this study, we analyzed the IHC studies performed for inflammatory and neoplastic skin disorders in cutaneous biopsies taken in our department during 1 year. From a total of 8579 skin biopsies performed throughout the year 2011 in our department, IHC studies were performed in 283 cutaneous biopsies. The total number of different antibodies used in the IHC studies of those 283 skin biopsies was 129. These antibodies were used in 1421 studies, with a mean of 5 cases per antibody studied. The proliferative marker MIB-1 was the single antibody with the highest number of studies, with a total of 119 (8.3% of all IHC studies performed), followed by 113 of CD3 (7.9% of total IHC studies) and 108 of Melan-A (7.6% of total IHC studies). Other hematopoietic differentiation markers, such as CD20, CD4, and CD8, and other melanocytic markers, such as S-100 protein, Melan-A, and HMB-45, were all investigated with a frequency greater than 50 studies each. The 2 most frequent categories were melanocytic neoplasms, which represented 25% of all specimens studied by IHC, and the proliferations of lymphohematopoietic nature, which were 20% of all studied samples and represented by far the highest number of IHC stains per case to reach a final diagnosis. Both previous categories together accounted for 45% of all diagnoses in which IHC was performed. We compare our results with the only similar study previously published in the literature. The gold standard panel of antibodies that should be available in everyday practice in dermatopathology to arrive at a specific diagnosis in each cutaneous inflammatory disease or neoplastic process involving the skin is still a matter of discussion.
机译：免疫组织化学（IHC）是一项辅助技术，可提高炎性和肿瘤性皮肤疾病的诊断准确性和组织病理学预后。但是，只有少数研究专门针对可用于炎症性或赘生性皮肤病的抗体集。在这项研究中，我们分析了我们部门在1年内针对皮肤活检中炎症性和赘生性皮肤疾病进行的IHC研究。在我们部门2011年全年进行的8579例皮肤活检中，对283例皮肤活检进行了IHC研究。在IHC研究中的那283次皮肤活检中使用的不同抗体的总数为129。这些抗体在1421次研究中使用，每个研究的抗体平均5例。增殖标记物MIB-1是研究次数最多的单一抗体，总数为119（占所有IHC研究的8.3％），其次是CD3的113（占IHC研究的7.9％）和Melan-108 A（占IHC研究总数的7.6％）。其他造血分化标记，例如CD20，CD4和CD8，以及其他黑素细胞标记，例如S-100蛋白，Melan-A和HMB-45，均以大于50个研究的频率进行了研究。 2个最常见的类别是黑素细胞性肿瘤，占IHC研究的所有标本的25％;淋巴造血性质的增殖，占所有研究标本的20％，是每个案例中IHC染色数量最高的标本。最终诊断。先前的两个类别合起来占进行IHC的所有诊断的45％。我们将我们的结果与先前在文献中发表的唯一类似研究进行比较。在皮肤病理学的日常实践中应该使用的金标准抗体小组，可以在涉及皮肤的每种皮肤炎性疾病或赘生性过程中做出特定的诊断，这仍然是一个讨论的问题。

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