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首页> 外文期刊>Journal of Parasitology >MOLECULAR CHARACTERIZATION OF HYPODERMA SPP. IN DOMESTIC RUMINANTS FROM TURKEY AND PAKISTAN
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MOLECULAR CHARACTERIZATION OF HYPODERMA SPP. IN DOMESTIC RUMINANTS FROM TURKEY AND PAKISTAN

机译：Hypoderma SPP的分子表征。 来自土耳其和巴基斯坦的国内反刍动物

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摘要

The aim of this study was to determine the morphological and molecular characterization of Hypoderma spp. in cattle and yak from provinces in Turkey and Pakistan. In total, 78 Hypoderma larvae were collected from slaughtered animals in Turkey and Pakistan from October 2015 to January 2016. Thirty-eight of these 78 Hypoderma larvae were morphologically classified as third instar larvae (L3s) of Hypoderma bovis, 37 were classified as Hypoderma lineatum, and 3 were classified as suspected or unidentified. The restriction enzyme TaqI was used to differentiate the Hypoderma spp. by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). According to the sequences and the PCR-RFLP results, all larval samples from cattle from Turkey were classified as H. bovis, except for 1 sample classified as H. lineatum. All Hypoderma larvae from Pakistan were classified as H. lineatum from cattle and as Hypoderma sinense from yak. This study provides the first molecular characterization of H. lineatum (cattle) and H. sinense (yak) in Pakistan based on PCR-RFLP and sequencing results.
机译：该研究的目的是确定皮下肌的形态和分子表征。从土耳其和巴基斯坦省的牛和牦牛。总共有78例皮下幼虫从土耳其和巴基斯坦从2015年10月到2016年1月收集。这78个皮下幼虫中的三十八个是幼稚植物的第三龄幼虫（L3s），37例被归类为Hypoderma lineatum。和3分为疑似或身份定值。限制酶Taqi用于区分Hypoderma SPP。通过聚合酶链反应（PCR） - 裂解片段长度多态性（RFLP）。根据序列和PCR-RFLP结果，从土耳其牛的所有幼虫样品被归类为Bovis，除了1种样品分类为H.Inainum。从巴基斯坦的所有Hypoderma幼虫被归类为牛的H.Inaidum，从牦牛那里作为Hypoderma Sinense。本研究提供了基于PCR-RFLP和测序结果的巴基斯坦H.Inainum（牛）和H.Inense（牦牛）的第一次分子表征。

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