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首页> 外文期刊>Journal of Parasitology >Development and Characterization of 12 Novel Polymorphic Microsatellite Loci for the Mammal Chewing Louse Geomydoecus aurei (Insecta: Phthiraptera) and a Comparison of Next-Generation Sequencing Approaches for Use in Parasitology
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Development and Characterization of 12 Novel Polymorphic Microsatellite Loci for the Mammal Chewing Louse Geomydoecus aurei (Insecta: Phthiraptera) and a Comparison of Next-Generation Sequencing Approaches for Use in Parasitology

机译：哺乳动物咀嚼虱子哺乳动物乳房（Inseta：Phthiralea）的12种新型多晶型微卫星基因座的开发和表征及其在寄生虫学中使用下一代测序方法的比较

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摘要

Next-generation sequencing methodologies open the door for evolutionary studies of wildlife parasites. We used 2 next-generation sequencing approaches to discover microsatellite loci in the pocket gopher chewing louse Geomydoecus aurei for use in population genetic studies. In one approach, we sequenced a library enriched for microsatellite loci; in the other approach, we mined microsatellites from genomic sequences. Following microsatellite discovery, promising loci were tested for amplification and polymorphism in 390 louse individuals from 13 pocket gopher hosts. In total, 12 loci were selected for analysis (6 from each methodology), none of which exhibited evidence of null alleles or heterozygote deficiencies. These 12 loci showed adequate genetic diversity for population-level analyses, with 3-9 alleles per locus with an average H-E per locus ranging from 0.32 to 0.70. Analysis of Molecular Variance (AMOVA) indicated that genetic variation among infrapopulations accounts for a low, but significant, percentage of the overall genetic variation, and individual louse infrapopulations showed F-ST values that were significantly different from zero in the majority of pairwise infrapopulation comparisons, despite all 13 infrapopulations being taken from the same locality. Therefore, these 12 polymorphic markers will be useful at the infrapopulation and population levels for future studies involving G. aurei. This study shows that next-generation sequencing methodologies can successfully be used to efficiently obtain data for a variety of evolutionary questions.
机译：下一代测序方法为野生动物寄生虫进化研究开门。我们使用了2个下一代测序方法来探索口袋流水咀嚼虱子地质yeCeCus的遗产的微卫星基因座，以用于人口遗传学研究。在一种方法中，我们测量了富含微卫星基因座的库;在另一种方法中，我们从基因组序列中开采微卫星。在微卫星发现之后，在来自13个口袋药物宿主的390个虱子个体中测试了有前途的基因座进行扩增和多态性。总共选择12个基因座进行分析（来自每种方法的6个），其中没有任何表现出零等位基因或杂合子缺乏的证据。这些11个基因座表明，人口水平分析的适当遗传多样性，每位基因座的3-9个等位基因，平均H-E每个基因座，范围为0.32至0.70。分子方差分析（AMOVA）表明侵占性遗传变异占整体遗传变异的低，但显着，百分比，单独的丙替换率显示出与大多数成对侵占比较的零显着不同的F-ST值尽管所有来自同一地点的所有13个违规行为。因此，这12种多态性标记物在侵占和人口水平下将有用，以涉及G. Aurei的未来研究。本研究表明，下一代测序方法可以成功地用于有效地获得各种进化问题的数据。

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  • 来源
    《Journal of Parasitology》 |2018年第1期|共7页
  • 作者

    Light J. E.; Harper S. E.; Johnson K. P.; Demastes J. W.; Spradling T. A.;

  • 作者单位

    Texas A&

    M Univ Dept Wildlife &

    Fisheries Sci 534 John Kimbrough Blvd College Stn TX 77843 USA;

    Texas A&

    M Univ Dept Wildlife &

    Fisheries Sci 534 John Kimbrough Blvd College Stn TX 77843 USA;

    Texas A&

    M Univ Dept Wildlife &

    Fisheries Sci 534 John Kimbrough Blvd College Stn TX 77843 USA;

    Texas A&

    M Univ Dept Wildlife &

    Fisheries Sci 534 John Kimbrough Blvd College Stn TX 77843 USA;

    Texas A&

    M Univ Dept Wildlife &

    Fisheries Sci 534 John Kimbrough Blvd College Stn TX 77843 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 寄生动物、寄生虫学;
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